KITLG

The gene "Kitlg" corresponds to the protein known as KIT ligand. Commonly, it's also called stem cell factor (SCF) and mast cell growth factor (MGF). KIT ligand binds to the receptor tyrosine kinase KIT, promoting its dimerization and autophosphorylation. This activates multiple downstream signal pathways, such as the PI3K - AKT and MAPK pathways.
In terms of disease relevance, abnormalities in KIT ligand or its receptor are linked to several disorders, including some hematological malignancies and piebaldism. Regarding drug development, agents targeting the KIT - KIT ligand axis are under investigation, aiming to modulate the abnormal signaling and treat associated diseases.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of MO7e cells is 1.813-3.203 ng/mL.

The purity of KITLG was greater than 95% as determined by SEC-HPLC

Recombinant Mouse Kit ligand (Kitlg), partial (Active)(CSB-MP012376MO1)

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA.Immobilized Mouse Kitlg at 2 μg/ml can bind Mouse Kit(CSB-MP012375MO). The EC₅₀ is 15.57-20.95 ng/mL.

Activity
The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is 1.691-11.94 ng/mL.

Recombinant Human Kit ligand (KITLG), partial (Active)(CSB-MP002061HU1i9)

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA.Immobilized Human KIT(CSB-MP012375HU1) at 2 μg/ml can bind Human KITLG. The EC₅₀ is 0.6921-0.8551 ng/mL.

KITLG Recombinant Monoclonal Antibody(CSB-RA183183A0HU)

Validated Data

Western Blot
Positive WB detected in: PC-3 whole cell lysate(30µg), HT-29 whole cell lysate(30µg), K562 whole cell lysate(30µg), Hela whole cell lysate(30µg), A549 whole cell lysate(30µg)
All lanes: SCF antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 31 kDa
Observed band size: 40 kDa
Exposure time：1min40s

IHC image of CSB-RA183183A0HU diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing 786-O cells stained with CSB-RA183183A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

The following KITLG reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

KITLG Antibodies

KITLG Proteins

KITLG ELISA Kits

KITLG Antibodies

KITLG Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA012376ESR2HU	KITLG Antibody	Human	ELISA, WB
CSB-PA122514	KITLG Antibody	Human	ELISA,WB
CSB-RA183183A0HU	KITLG Recombinant Monoclonal Antibody	Human	ELISA, WB, IHC, FC

KITLG Proteins

KITLG Proteins for Ovis aries (Sheep)

Code	Product Name	Source
CSB-CF012376SH	Recombinant Sheep Kit ligand (KITLG)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-YP012376SH1 CSB-EP012376SH1 CSB-BP012376SH1 CSB-MP012376SH1 CSB-EP012376SH1-B	Recombinant Sheep Kit ligand (KITLG), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

KITLG Proteins for Gallus gallus (Chicken)

Code	Product Name	Source
CSB-YP012376CH CSB-EP012376CH CSB-BP012376CH CSB-MP012376CH CSB-EP012376CH-B	Recombinant Chicken Kit ligand (KITLG), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

KITLG Proteins for Equus caballus (Horse)

Code	Product Name	Source
CSB-YP012376HO CSB-EP012376HO CSB-BP012376HO CSB-MP012376HO CSB-EP012376HO-B	Recombinant Horse Kit ligand (KITLG), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

KITLG Proteins for Felis catus (Cat) (Felis silvestris catus)

Code	Product Name	Source
CSB-CF012376CA	Recombinant Cat Kit ligand (KITLG)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-YP012376CA1 CSB-EP012376CA1 CSB-BP012376CA1 CSB-MP012376CA1 CSB-EP012376CA1-B	Recombinant Cat Kit ligand (KITLG), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

KITLG Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-CF012376RA	Recombinant Rat Kit ligand (Kitlg)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-YP012376RA1 CSB-EP012376RA1 CSB-BP012376RA1 CSB-MP012376RA1 CSB-EP012376RA1-B	Recombinant Rat Kit ligand (Kitlg), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

KITLG Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-EP012376HU	Recombinant Human Kit ligand (KITLG), partial	E.coli
CSB-AP002061HU	Recombinant Human Kit ligand protein (KITLG), partial (Active)	E.coli
CSB-CF012376HU(A4)	Recombinant Human Kit ligand (KITLG)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-AP003741HU	Recombinant Human Kit ligand (KITLG), partial (Active)	E.coli
CSB-AP003881HU	Recombinant Human Kit ligand (KITLG), partial (Active)	Mammalian cell
CSB-MP002061HU	Recombinant Human Kit ligand (KITLG), partial	Mammalian cell
CSB-MP002061HU1	Recombinant Human Kit ligand (KITLG), partial (Active)	Mammalian cell
CSB-MP002061HU1i9	Recombinant Human Kit ligand (KITLG), partial (Active)	Mammalian cell

KITLG Proteins for Sus scrofa (Pig)

Code	Product Name	Source
CSB-YP012376PI CSB-EP012376PI CSB-BP012376PI CSB-MP012376PI CSB-EP012376PI-B	Recombinant Pig Kit ligand (KITLG), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

KITLG Proteins for Coturnix japonica (Japanese quail) (Coturnix coturnix japonica)

Code	Product Name	Source
CSB-YP012376DXJ CSB-EP012376DXJ CSB-BP012376DXJ CSB-MP012376DXJ CSB-EP012376DXJ-B	Recombinant Coturnix coturnix japonica Kit ligand (KITLG), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

KITLG Proteins for Bos taurus (Bovine)

Code	Product Name	Source
CSB-YP012376BO CSB-EP012376BO CSB-BP012376BO CSB-MP012376BO CSB-EP012376BO-B	Recombinant Bovine Kit ligand (KITLG), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP012376BO	Recombinant Bovine Kit ligand (KITLG), partial	E.coli

KITLG Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-AP003151MO	Recombinant Mouse Kit ligand protein (Kitlg), partial (Active)	E.coli
CSB-CF012376MO	Recombinant Mouse Kit ligand (Kitlg)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-YP012376MO1 CSB-EP012376MO1 CSB-BP012376MO1 CSB-MP012376MO1 CSB-EP012376MO1-B	Recombinant Mouse Kit ligand (Kitlg), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-AP004071MO	Recombinant Mouse Kit ligand (Kitlg), partial (Active)	E.coli
CSB-MP012376MO1	Recombinant Mouse Kit ligand (Kitlg), partial (Active)	Mammalian cell
CSB-YP012376MO1	Recombinant Mouse Kit ligand (Kitlg), partial	Yeast
CSB-MP012376MO1d9	Recombinant Mouse Kit ligand (Kitlg), partial (Active)	Mammalian cell

KITLG Proteins for Neovison vison (American mink) (Mustela vison)

Code	Product Name	Source
CSB-YP012376MVE CSB-EP012376MVE CSB-BP012376MVE CSB-MP012376MVE CSB-EP012376MVE-B	Recombinant Mustela vison Kit ligand (KITLG), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

KITLG Proteins for Capra hircus (Goat)

Code	Product Name	Source
CSB-YP012376GO CSB-EP012376GO CSB-BP012376GO CSB-MP012376GO CSB-EP012376GO-B	Recombinant Goat Kit ligand (KITLG), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli