Abbreviation
Recombinant Mouse Kitlg protein, partial (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 10 ng/ml, corresponding to a specific activity of >1.0x105 IU/mg.
Alternative Names
Kitlg; Kitl; Mgf; Sl; Slf; Kit ligand; Hematopoietic growth factor KL; Mast cell growth factor; MGF; Steel factor; Stem cell factor; SCF; c-Kit ligand
Species
Mus musculus (Mouse)
Expression Region
26-189aa
Target Protein Sequence
M+KEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Stem cell factor (SCF) is a critical ligand for the c-Kit receptor tyrosine kinase, driving hematopoietic progenitor proliferation, mast cell development, and melanocyte survival through activation of downstream MAPK and JAK/STAT signaling cascades. This tag-free recombinant mouse Kitlg (residues 26–189) encompasses the mature extracellular domain and demonstrates strong signaling potency, with an ED50 below 10 ng/ml in TF-1 cell proliferation assays corresponding to a specific activity exceeding 1.0 × 10⁵ IU/mg. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts, making this protein appropriate for sensitive immune cell functional assays—including mast cell differentiation, hematopoietic progenitor expansion, and c-Kit/MAPK pathway phosphorylation studies—as well as in vivo murine models of hematopoiesis or tumor biology. Purity exceeding 97% by SDS-PAGE further supports use as a reliable reference standard in SCF detection assays or as a positive control for antibody validation by ELISA and western blot.
