Vascular endothelial growth factor A (VEGFA), also known as vascular permeability factor (VPF), is a crucial protein in angiogenesis. It binds to receptors VEGFR - 1 and VEGFR - 2 on endothelial cells, triggering a cascade of intracellular signals. Activation of these receptors promotes endothelial cell proliferation, migration, and survival, and enhances vascular permeability.
VEGFA is involved in various diseases such as cancer, where it promotes tumor angiogenesis, and age - related macular degeneration. In drug development, anti - VEGFA agents like bevacizumab have been used clinically to block VEGFA - mediated angiogenesis, showing significant efficacy in treating certain cancers and eye diseases, with continuous efforts to develop more targeted and efficient therapies.
Popular
Product:
Recombinant Human Vascular endothelial growth factor A, long form (VEGFA) (Active)(CSB-MP025833HU(F4))
Validated Data
-SDS.jpg)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
-AC1.jpg)
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human VEGFA protein at 2 μg/ml can bind Anti-VEGFA recombinant antibody (CSB-RA025833MA2HU). The EC50 is 0.6797-0.8382 ng/mL.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human FLT1 (CSB-MP008732HU) protein at 2 μg/ml can bind Human VEGFA protein. The EC50 is 28.72-33.22 ng/mL.
VEGFA Recombinant Monoclonal Antibody(CSB-RA025833MA1HU)
Validated Data
IHC image of CSB-RA025833MA1HU diluted at 1:50 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of hela cell with CSB-RA025833MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of MCF7 cell with CSB-RA025833MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The following VEGFA
reagents supplied by CUSABIO are manufactured under a
strict quality control system. Multiple applications
have been validated and solid technical support is
offered.
VEGFA Proteins
VEGFA Proteins for Mesocricetus auratus (Golden hamster)
VEGFA Proteins for Canis lupus familiaris (Dog) (Canis familiaris)
VEGFA Proteins for Ovis aries (Sheep)
VEGFA Proteins for Gallus gallus (Chicken)
VEGFA Proteins for Equus caballus (Horse)
VEGFA Proteins for Homo sapiens (Human)
VEGFA Proteins for Rattus norvegicus (Rat)
VEGFA Proteins for Coturnix japonica (Japanese quail) (Coturnix coturnix japonica)
VEGFA Proteins for Sus scrofa (Pig)
VEGFA Proteins for Bos taurus (Bovine)
VEGFA Proteins for Mus musculus (Mouse)
VEGFA Proteins for Cavia porcellus (Guinea pig)
