Pig Vascular Endothelial cell Growth Factor,VEGF ELISA KIT

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Pig Vascular Endothelial cell Growth Factor,VEGF ELISA KIT

Name: Pig Vascular Endothelial cell Growth Factor,VEGF ELISA KIT
Brand: CUSABIO
SKU: CSB-E12053p

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Code: CSB-E12053p

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Citation （7）

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Size:

96T,5×96T,10×96T

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Sample Types: serum, plasma, tissue homogenates

Detect Range: 4.69 pg/mL-300 pg/mL

Sensitivity: 1.17 pg/mL

Trial Size:

24T ELISA Kit Trial Size (Only USD$150/ kit)
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Alternative Names

VEGFA ELISA kit; VEGFVascular endothelial growth factor A ELISA kit; VEGF-A ELISA kit; Vascular permeability factor ELISA kit; VPF ELISA kit

Abbreviation

VEGFA

Uniprot NO.

P49151

Species

Sus scrofa (Pig)

Sample Type

serum, plasma, tissue homogenates

Detection Range

4.69 pg/mL-300 pg/mL

Sensitivity

1.17 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Measurement

Sandwich

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	92
	Range %	85-98
1:10	Average %	90
	Range %	85-97
1:20	Average %	96
	Range %	89-103
1:40	Average %	86
	Range %	81-92

Recovery

The recovery of pig VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	96	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
300	2.032	1.984	2.008	1.871
150	1.746	1.744	1.745	1.608
75	1.465	1.487	1.476	1.339
37.5	1.109	1.127	1.118	0.981
18.75	0.763	0.736	0.750	0.613
9.37	0.525	0.521	0.523	0.386
4.69	0.334	0.348	0.341	0.204
0	0.135	0.138	0.137

Troubleshooting and FAQs

ELISA kit FAQs

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Description

Vascular endothelial growth factor A (VEGFA) is a key signaling protein that regulates angiogenesis, the formation of new blood vessels from existing vasculature. This growth factor binds to specific receptors on endothelial cells, promoting their proliferation, migration, and survival—processes that are essential in both physiological and pathological angiogenesis. VEGFA plays important roles in embryonic development, wound healing, and tissue repair. Its dysregulation is implicated in various diseases including cancer, diabetic retinopathy, and cardiovascular disorders. Research into VEGFA signaling pathways has provided valuable insights into therapeutic targets for anti-angiogenic treatments.

The Pig Vascular Endothelial cell Growth Factor VEGF ELISA KIT (CSB-E12053p) is designed for quantitative measurement of VEGFA in Sus scrofa samples. This sandwich ELISA works with serum, plasma, and tissue homogenates with a detection range of 4.69 pg/mL to 300 pg/mL and sensitivity of 1.17 pg/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours, with detection performed at 450 nm wavelength.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in research studies to quantify vascular endothelial growth factor A levels in cell culture supernatants. The applications show its use in measuring secreted protein concentrations from cultured cells under various experimental conditions.

• Cell culture studies: Measurement of vascular endothelial growth factor A secretion from cultured cells to determine protein production and release
• Biomarker quantification: Analysis of culture media to determine concentrations of secreted growth factors in controlled experimental settings
• Protein expression research: Analysis of cellular responses through quantitative measurement of growth factor levels in culture supernatants

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function(From Uniprot)

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development.

Gene References into Functions

It regulates endometrial remodeling in the porcine endometrial tissues during follicular and luteal phase. PMID:28139071
It was concluded that VEGF and factor VIII are important growth factors associated with fetal development in pigs and are identified in all uterine segments. PMID:28237345
results are consistent with a participation of (VEFG) in the regulation of the dynamics of oviductal fluid secretion and the oviduct contractibility PMID:28257866
Here we demonstrate that VEGF-165 mediates MSC differentiation into ECs via VEGFR-2-dependent induction of Sox18, which ultimately coordinates the transcriptional upregulation of specific markers of the EC phenotype. PMID:25913202
VEGF was significantly downregulated 7 days after cryotherapy of the sclera and stayed at that level until day 14. It returned to baseline by day 21. PMID:25510786
VEGF production, blood vessel network and follicle remodeling are impaired by the antiprogesterone RU486. PMID:24756033
findings suggest that the augmented neovascular response seen with VEGF administration was through the VEGF-induced upregulation of Notch signaling. PMID:24366099
interleukin-1beta-induced vascular endothelial growth factor in airway smooth muscle cells PMID:24142467
Upregulation of VEGF during hypoxia in chondrocyte is mediated partially through HIF-1alpha. PMID:22970342
data shows that members of the VEGF-VEGFR system are temporally and spatially well localized for playing key roles during umbilical cord formation and its intensive growth observed after day 75 of pregnancy PMID:20825582
cardiac-specific and hypoxia-induced coexpression of VEGF and Ang1 improves the perfusion and function of porcine MI heart through the induction of angiogenesis and cardiomyocyte proliferation PMID:21245320
The mRNA for VEGFA was expressed in early developing and in maturing glomeruli. PMID:20713984
Data show that subventricular zone neural stem cells express VEGF and all VEGF splice variants, VEGFR1, VEGFR2 and Neuropilin-1 and -2 mRNAs. PMID:20552272
The earlier increase in VEGF protein and mRNA expression in the Meishan versus the Yorkshire conceptus may explain the previously reported increased vascularity and increased placental efficiency of this breed compared the Yorkshire breed. PMID:14998908
Contrast media did not affect bone marrow cell viability/VEGF secretion. PMID:15274157
VEGF upregulation in the proliferative zone after ischemic damage may play a role in stimulating vascular invasion and granulation tissue formation in the necrotic hypertrophic zone of the epiphyseal cartilage PMID:15537448
Data demonstrate that lipopolysaccharides evoke a heat shock response, with an increase heat shock proteins 70 and Hsp32) and of VEGF, a specific endothelial cell growth factor. PMID:16333987
Vascular endothelial growth factor (VEGF) plays an important role in the thecal angiogenesis during follicular development. PMID:16372043
number of preovulatory follicles and the capillary density in the theca interna increased significantly in the ovaries injected with VEGF gene PMID:16538032
our study revealed the presence of VEGF in endothelial and smooth muscle cells of vascular subovarian plexus arteries PMID:16584087
Differential regulation of VEGF isoforms may suggest specific physiological roles for some of them, particularly in angioregression occurring during the apoptotic structural luteolysis. PMID:16967516
VEGF ligand-receptor system may play an important role in the development and maintenance of the corpus luteum in pigs. PMID:17120307
Sanguinarine inhibited both VEGF production and VEGF-induced Akt activation in swine granulosa cells. PMID:17310078
VEGF/Flk-1/Flt-1 system is activated during myocardial ischemia reperfusion injury. PMID:17868880
VEGF-A family members are probably involved in appropriate preparation of endometrium for implantation and in vascular events during implantation in pigs. PMID:17874446
Hemodialysis graft placement leads to early increases in wall shear stress, VEGF-A, pro-MMP-9, MMP-2, VEGFR-1, VEGFR-2, and TIMP-1, which may contribute to the development of venous stenosis. PMID:18326810
Interaction of soluble factors in plasma possibly generated during PICSO are not responsible for upregulation of HO-1 and VEGF mRNA. PMID:19618599