GLP1R

Recombinant Human Glucagon-like peptide 1 receptor (GLP1R), partial (Active)(CSB-MP009514HUb1)

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human GLP1R at 2 μg/mL can bind Anti-GLP1R recombinant antibody (CSB-RA009514MA1HU), the EC₅₀ is 54.54-94.23 ng/mL.

Recombinant Mouse Glucagon-like peptide 1 receptor (Glp1r), partial (Active)(CSB-MP009514MO1)

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Mouse Glp1r at 2 μg/ml can bind Anti-GLP1R recombinant antibody (CSB-RA009514MA3HU). The EC₅₀ is 141.7-172.4 ng/mL.

Recombinant Macaca fascicularis Glucagon-like peptide 1 receptor (GLP1R), partial (Active)(CSB-MP4826MOV)

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis GLP1R at 2 μg/ml can bind Anti-GLP1R recombinant antibody (CSB-RA009514MA2HU). The EC₅₀ is 1.292-1.518 ng/mL.

Recombinant Rat Glucagon-like peptide 1 receptor (Glp1r), patial (Active)(CSB-MP009514RA2)

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Rat Glp1r at 2 μg/ml can bind Anti-GLP1R recombinant antibody (CSB-RA009514MA2HU). The EC₅₀ is 8.630-10.66 ng/mL.

GLP1R Recombinant Monoclonal Antibody(CSB-RA009514MA1HU)

Validated Data

The Binding Activity of GLP1R with anti-GLP1R antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human GLP1R(CSB-MP009514HUb1) at 2 μg/mL can bind Anti-GLP1R recombinant antibody, the EC₅₀ is 54.54-94.23 ng/mL.

Untransfected HEK-293T cells surface (green line) and transfected Human GLP1R HEK-293T stable cells surface (red line) were stained with anti-GLP1R antibody (2µg/1*10⁶cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

Positive WB detected in: MCF-7 whole cell lysate(30µg),Mouse Heart tissue lysate(30µg),Rat Heart tissue lysate(30µg)
All lanes: GLP1R antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 300s

GLP1R Antibody(CSB-PA009514YA01HU)

Validated Data

Western Blot
Positive WB detected in: U87 whole cell lysate(30µg), SHSY5Y whole cell lysate(30µg), 293T whole cell lysate(30µg), COLO205 whole cell lysate(30µg), Hela whole cell lysate(30µg), JK whole cell lysate(30µg),Mouse Heart tissue lysate(30µg),Mouse Brain tissue lysate(30µg)
All lanes: GLP1R antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 120s

Immunofluorescence staining of HepG2 cell with CSB-PA009514LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).