CLDN6

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 at 2 μg/ml can bind Anti-CLDN6/9 recombinant antibody (CSB-RA005508MA1HU). The EC₅₀ is 1.243-2.426 ng/mL.

Activity
Human CLDN6 Monoclonal Antibody (CSB-RA005508MA1HU) captured on Protein A Chip can bind Human CLDN6 Full Length Protein with an affinity constant of 5.43 nM as detected by MetaSPR Assay(in presence of DDM/CHS) (WeSPR^TM 200).

Recombinant Human Claudin-6 (CLDN6), partial-VLPs

CLDN6/9 Recombinant Monoclonal Antibody

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA005508LA01HU at dilution of 1:100

Immunofluorescence staining of HepG2 cells with CSB-PA005508LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).