N Recombinant Monoclonal Antibody

Code: CSB-RA33255A2GMY

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Size:

50ul100ul

US$210

Quantity:

Species Reactivity: Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Application: ELISA, GICA

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Target Background

Product Details

Uniprot NO.

P0DTC9

Alternative Names

N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N

Species Reactivity

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Immunogen

Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (CSB-EP3325GMY)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Mouse scFv fusion with human IgG1 Fc

Clone No.

4D2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol

Recommended Dilution

Application	Recommended Dilution
ELISA	1:10000-1:50000
GICA	1:500-1:5000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N
Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC₅₀ is 2.928 ng/ml.
In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (CSB-RA33255A2GMY) is clean, the detection limit can be as low as 15.625ng/ml (1.09ng/0.07ml), and the sensitivity is very good.

Description

The SARS-CoV-2 nucleocapsid protein serves as one of the most abundant viral proteins during infection, playing essential roles in viral RNA packaging, replication, and host cell interactions. Its high immunogenicity and conservation across viral variants have established it as a primary target for COVID-19 diagnostic development and seroprevalence studies, making reliable detection reagents critical for ongoing coronavirus research.

This recombinant monoclonal antibody targeting the SARS-CoV-2 nucleocapsid protein offers researchers the consistency and reproducibility that recombinant technology provides. Generated against the full-length nucleoprotein sequence spanning amino acids 1-419, the antibody is produced as a mouse scFv fusion with human IgG1 Fc, combining the specificity of monoclonal selection with a defined molecular format. Because the antibody sequence is fixed and production occurs in controlled expression systems, researchers can expect uniform performance across different lots, eliminating the variability concerns that can complicate longitudinal studies or assay development workflows.

Validation data demonstrate exceptional binding characteristics, with functional ELISA testing revealing an EC50 of 2.928 ng/ml when binding to immobilized SARS-CoV-2 nucleocapsid protein. For rapid detection applications, colloidal gold immunochromatography assay testing shows clean background signal with detection sensitivity reaching as low as 15.625 ng/ml, supporting its utility in point-of-care or high-throughput screening contexts. The recommended working dilutions span 1:10000 to 1:50000 for ELISA and 1:500 to 1:5000 for immunochromatographic applications, offering flexibility to optimize signal-to-noise ratios across different experimental setups.

This antibody supports researchers developing diagnostic platforms, studying viral protein biology, or investigating host-pathogen interactions in the context of SARS-CoV-2 infection.

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Target Background

Function(From Uniprot)

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.

Subcellular Location

Virion. Host cytoplasm.

Protein Families

Betacoronavirus nucleocapsid protein family

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