N Recombinant Monoclonal Antibody

CSB-RA33255A2GMY

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50ul100ul

US$210

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Species Reactivity: Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Application: ELISA, GICA

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Target Background

Product Details

Uniprot NO.

P0DTC9

Alternative Names

N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N

Species Reactivity

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Immunogen

Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (CSB-EP3325GMY)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Mouse scFv fusion with human IgG1 Fc

Clone No.

4D2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Scope Application

ELISA, GICA

Recommended Dilution

Application	Recommended Dilution
ELISA	1:10000-1:50000
GICA	1:500-1:5000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Protocols

ELISA Protocol

Datasheet & COA

Antibody FAQs

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N Recombinant Monoclonal Antibody

Description

The generation of the N recombinant monoclonal antibody involves a rigorous process to ensure its exceptional quality and specificity. Firstly, B cells are isolated from the spleen of an immunized animal, with the recombinant human SARS-CoV-2 N protein (1-419aa) used as the immunogen. RNA is extracted from the B cells and converted into cDNA through reverse transcription. The N antibody genes are then amplified using specific primers designed for the antibody constant regions and cloned into an expression vector. The human IgG1 Fc is inserted into the vector, downstream of the N antibody. This recombinant vector is transfected into host cells to enable the production of the N recombinant monoclonal antibody. After a period of cell culture, the antibody is collected from the cell culture supernatant and purified using affinity chromatography, resulting in a highly purified form suitable for various applications. To ensure its quality and functionality, the antibody undergoes thorough characterization through assays including ELISA and GICA which validate its specificity and ability to detect human SARS-CoV-2 N protein. The meticulous production process guarantees the development of a reliable and effective N recombinant monoclonal antibody, essential for a wide range of human SARS-CoV-2-related research.

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Target Background

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.

Subcellular Location

Virion. Host cytoplasm.

Protein Families

Betacoronavirus nucleocapsid protein family

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