Human SARS-CoV-2 N IgG Antibody ELISA Kit

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Code: CSB-EL3325HU
Size:
96T,5×96T,10×96T
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Quantity:
Sample Types: serum, plasma
Trial Size:
24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>
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Product Details

Alternative Names
N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
Abbreviation
SARS-CoV-2 N Ab (IgG)
Uniprot NO.
Species
Homo sapiens (Human)
Sample Type
serum, plasma
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Infectious Diseases
Assay Principle
qualitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Measurement
Indirect
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Precision
Intra-assay Precision (Precision within an assay): CV%<15%  
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%  
Three samples of known concentration were tested in twenty assays to assess.
Typical Data

Test parameter

specification

test result

Positive control

>0.8

0.967

Negative control

<0.2

0.184

Positive rate

20,Positive

100%

Negative rate

20,Negative

100%

Materials provided
    • A 96-well Coated assay plate --This microplate has been pre-coated with human SARS-CoV-2 nucleoprotein.
    • Negative Control (1 x 800 μl) --It is free of the SARS-CoV-2 N IgG antibody and used to preclude the false positive.
    • Positive Control (1 x 800 μl) --Used to evaluate the validity, stability, and comparability of the test results.
    • HRP-conjugated anti-Human IgG antibody (100 x concentrate) (1 x 120 μl) --Act as the detection antibody.
    • HRP-conjugate Diluent (1 x 20 ml) --Dilute the HRP-conjugated anti-Human IgG antibody.
    • Sample Diluent (2 x 20 ml) --Dilute the sample solution.
    • Wash Buffer (25 x concentrate) (1 x 20 ml) --Wash the unbound reagents.
    • TMB Substrate (1 x 10 ml) --React with HRP, eliciting a chromogenic color reaction.
    • Stop Solution (1 x 10 ml) --Stop the color reaction. The solution turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --Seal the microplate when incubation.
    • An Instruction manual

Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Materials not provided
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer.
    • Absorbent paper for blotting the microtiter plate.
    • 50-300ul multi-channel micropipette
    • 100ml and 500ml graduated cylinders.
    • Deionized or distilled water.
    • Pipette tips
    • Timer
    • Test tubes for dilution.

Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Troubleshooting and FAQs
Description

CUSABIO's Human SARS-CoV-2 nucleoprotein (N) IgG Antibody ELISA Kit allows for in vitro qualitative detection of the IgG antibody-specific for SARS-CoV-2 N protein in human serum and plasma. This research-use only kit can be applied in the studies associated with COVID-19, infectious and severe pneumonia caused by SARS-CoV-2. It has been validated with high sensitivity, high specificity, and high precision.

Nucleoprotein is the most abundant protein in SARS-CoV-2. It packs the positive-sense single-stranded viral RNA into a helical ribonucleocapsid (RNP), forming the viral nucleocapsid (N). The N protein plays an important role in improving the efficiency of virus transcription and assembly. It is also involved in viral pathogenesis. N protein possessing an immunogenic effect makes it trigger an immune response, which leads to the production of IgM/G against SARA-CoV-2 N protein. SARS-CoV-2 N IgG antibodies appear in the serum after 2-3 weeks after the onset of the symptoms, peaks within the third week, and gradually declines to a lower level. IgG-specific for SARS-CoV-2 N protein rapidly increases to a higher level after a secondary infection and retains in the body for several months even years.

Customer Reviews and Q&A

 Customer Reviews
 Q&A
Q:

How do you use the Elisa kit using nucleoprotein or spike protein? Is there any difference in the recommended purpose fo usage?
Also, which do you recommend, IgG or IgM detection for CoVID-19?

A:

Regarding ELISA kit for the detection of CoVID-19, we could only provide this item: CSB-EL3325HU, Human Novel Coronavirus Nucleoprotein (SARS-CoV-2 N) IgG Antibody ELISA Kit. It is only for research use, not for diagnostic purposes.
Usually, IgM is generated first in the primary immune response. IgM appears first in the process of virus infection, but it doesn't last long; IgG is the main antibody produced by the re-immune response, and it lasts for a long time. Generally, it indicates a past infection.

Target Background

Function(From Uniprot)
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
Subcellular Location
Virion. Host cytoplasm.
Protein Families
Betacoronavirus nucleocapsid protein family
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