N Recombinant Monoclonal Antibody, Biotin conjugated

Code: CSB-RA33255D1GMY

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Size:

50ul100ul

US$210

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Species Reactivity: Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Application: ELISA

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Product Details
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Target Background

Product Details

Uniprot NO.

P0DTC9

Target Names

N (Nucleoprotein)

Alternative Names

N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N

Species Reactivity

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Immunogen

Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)

Immunogen Species

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Conjugate

Biotin

Clonality

Monoclonal

Isotype

Mouse scFv fusion with human IgG1 Fc

Clone No.

1A6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA

Protocols

ELISA Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Description

The production of the N recombinant monoclonal antibody is carried out through a systematic and thorough process to ensure its quality and specificity. The journey begins by isolating B cells from the spleen of an immunized animal, where the recombinant human SARS-CoV-2 N protein (1-419aa) is utilized as the immunogen. The RNA is then extracted from these B cells and converted into cDNA through reverse transcription. Using specific primers designed for the antibody constant regions, the N antibody genes are amplified and inserted into an expression vector. The human IgG1 Fc is integrated into the vector, downstream of the N antibody gene. The biotin is also inserted into the vector. The recombinant vector is then introduced into host cells through transfection, enabling the production of the N recombinant monoclonal antibody. Following an appropriate incubation period, the antibody is collected from the cell culture supernatant and subjected to purification using affinity chromatography, ensuring a high level of purity. To validate its specificity and functionality in detecting human SARS-CoV-2 N protein, the purified N recombinant monoclonal antibody undergoes rigorous characterization through ELISA. This meticulous production process yields a reliable and effective N recombinant monoclonal antibody, serving as a valuable tool in various research related to SARS-CoV-2.

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Target Background

Function(From Uniprot)

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.

Subcellular Location

Virion. Host cytoplasm.

Protein Families

Betacoronavirus nucleocapsid protein family

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