N Recombinant Monoclonal Antibody, HRP conjugated

CSB-RA33255B1GMY

Rate （N/A）
Review （0）

Size:

50ul100ul

US$210

Quantity:

Species Reactivity: Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Application: ELISA

Send an Inquiry

Start an on-line Chat

Orders can only be placed from within the U.S.

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot NO.

P0DTC9

Alternative Names

N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N

Species Reactivity

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Immunogen

Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) CSB-EP3325GMY

Conjugate

HRP

Clonality

Monoclonal

Isotype

Mouse scFv fusion with human IgG1 Fc

Clone No.

1A6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Scope Application

ELISA

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Protocols

ELISA Protocol

Datasheet & COA

Antibody FAQs

Images

N Recombinant Monoclonal Antibody, HRP conjugated

Description

The production of the N recombinant monoclonal antibody involves a systematic process to ensure its high quality and specific binding properties. The journey begins by isolating B cells from the spleen of an immunized animal, where the recombinant human SARS-CoV-2 N protein (1-419aa) is used as the immunogen. RNA is then extracted from these B cells and converted into cDNA through reverse transcription. The N antibody genes are amplified using specific primers designed for the antibody constant regions and cloned into an expression vector. The human IgG1 Fc is inserted into the vector, downstream of the N antibody gene and the HRP is also incorporated into the vector. This recombinant vector is introduced into host cells through transfection, allowing for the production of the N recombinant monoclonal antibody. After an appropriate incubation period, the antibody is collected from the cell culture supernatant and purified using affinity chromatography to ensure a highly pure form. The purified N recombinant monoclonal antibody is characterized using ELISA analysis to validate its specificity and functionality in detecting human SARS-CoV-2 N protein. Through this comprehensive production process, a reliable and effective N recombinant monoclonal antibody is generated, making it an invaluable tool for various SARS-CoV-2-associated research.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.

Subcellular Location

Virion. Host cytoplasm.

Protein Families

Betacoronavirus nucleocapsid protein family

Promotions