N Recombinant Monoclonal Antibody, HRP conjugated

Code: CSB-RA33255B1GMY

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50ul100ul

US$210

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Species Reactivity: Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Application: ELISA

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Target Background

Product Details

Uniprot NO.

P0DTC9

Target Names

N (Nucleoprotein)

Alternative Names

N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N

Species Reactivity

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Immunogen

Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) CSB-EP3325GMY

Immunogen Species

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Conjugate

HRP

Clonality

Monoclonal

Isotype

Mouse scFv fusion with human IgG1 Fc

Clone No.

1A6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA

Protocols

ELISA Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

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The Binding Activity of SARS-CoV-2-N Antibody-N Antibody, HRP conjugated with SARS-CoV-2-N
Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N (CSB-EP3325GMY) at 2 μg/ml can bind SARS-CoV-2-N Antibody, HRP conjugated, the EC₅₀ is 188.35 ng/ml.

Description

The SARS-CoV-2 nucleocapsid protein serves as one of the most abundant viral proteins during infection, playing essential roles in viral RNA packaging, replication, and host cell interactions. Its high immunogenicity and conservation across viral variants have established it as a primary target for COVID-19 diagnostics, seroprevalence studies, and investigations into viral pathogenesis.

This recombinant monoclonal antibody targeting the SARS-CoV-2 nucleocapsid protein offers researchers the reproducibility and consistency that recombinant technology provides. As a sequence-defined reagent produced from a single clone, it eliminates the lot-to-lot variability that can compromise longitudinal studies or assay development efforts. The engineered format combines a mouse single-chain variable fragment with a human IgG1 Fc domain, creating a well-characterized binding molecule with predictable behavior across experiments.

The direct HRP conjugation streamlines immunoassay workflows by eliminating secondary antibody incubation steps, reducing protocol time and potential background signal. Functional ELISA validation demonstrates strong binding activity against recombinant SARS-CoV-2 nucleoprotein, with an EC50 of 188.35 ng/ml when tested against immobilized antigen. The recommended working dilution range of 1:10,000 to 1:50,000 reflects the conjugate's sensitivity, allowing researchers to optimize signal-to-noise ratios for their specific assay conditions.

Supplied in a glycerol-based buffer system for stability at frozen storage temperatures, this antibody is well-suited for researchers developing or performing ELISA-based detection of SARS-CoV-2 nucleocapsid protein in diagnostic applications, vaccine response monitoring, or basic virology research exploring coronavirus biology and host-pathogen interactions.

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Target Background

Function(From Uniprot)

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.

Subcellular Location

Virion. Host cytoplasm.

Protein Families

Betacoronavirus nucleocapsid protein family

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