N Recombinant Monoclonal Antibody

Code: CSB-RA33255A0GMY

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Size:

50ul100ul

US$320

Quantity:

Species Reactivity: Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Application: ELISA, GICA

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Product Details
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Target Background

Product Details

Uniprot NO.

P0DTC9

Target Names

N (Nucleoprotein)

Alternative Names

N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N

Species Reactivity

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Immunogen

Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)(CSB-EP3325GMY)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Monoclonal mouse (varialbe region)/human (kappa/IgG1 constant)chimeric antibody

Clone No.

1A6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol

Recommended Dilution

Application	Recommended Dilution
ELISA	1:10000-1:50000
GICA	1:500-1:2000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N
Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N (CSB-EP3325GMY) at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC₅₀ is 6.892 ng/ml.
In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (CSB-RA33255A0GMY) is clean, the detection limit can be as low as 446.4ng/ml (31.25ng/0.07ml), and the sensitivity is very good.
ELISA: Immobilize various types of SARS proteins at concentration of 2μg/ml on solid substrate, then react with SARS-CoV-2-N Antibody at concentration of 100μg/ml, 10μg/ml and 1μg/ml. It shows the SARS-CoV-2-N Antibody (CSB-RA33255A0GMY) is specific for SARS-CoV-2-N protein, without any cross-reactivity with NL63-CoV, HCoV-OC43, HCoV-229E or HCoV-HKU1.

Description

The SARS-CoV-2 nucleocapsid protein serves as one of the most abundant viral proteins during infection, playing essential roles in viral RNA packaging, replication, and host cell interactions. Its high immunogenicity and conservation make it a primary target for diagnostic development and fundamental coronavirus research, complementing spike protein-focused studies with insights into viral assembly and pathogenesis.

This recombinant monoclonal antibody targeting the SARS-CoV-2 nucleocapsid protein offers the reproducibility and consistency that diagnostic and research applications demand. As a chimeric construct combining mouse variable regions with human kappa and IgG1 constant domains, it provides sequence-defined performance that eliminates the lot-to-lot variability inherent in traditional hybridoma production. The antibody was raised against a recombinant nucleoprotein construct spanning amino acids 1-419, ensuring recognition of properly folded epitopes.

Validation data demonstrate exceptional performance characteristics across immunoassay platforms. In functional ELISA, the antibody achieves an EC50 of 6.892 ng/ml, indicating high-affinity binding suitable for sensitive detection work. The recommended ELISA dilution range of 1:10000 to 1:50000 reflects this potency. For rapid testing applications, colloidal gold immunochromatography assay validation shows clean background signal with detection limits reaching 446.4 ng/ml, supporting point-of-care diagnostic development. Critically, specificity testing confirms no cross-reactivity with nucleocapsid proteins from related human coronaviruses including NL63-CoV, HCoV-OC43, HCoV-229E, and HCoV-HKU1, ensuring reliable discrimination of SARS-CoV-2 in samples where multiple coronavirus exposures may be relevant.

This antibody supports researchers developing SARS-CoV-2 diagnostic platforms, studying viral protein biology, or requiring a reliable detection reagent for nucleocapsid-focused coronavirus investigations.

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Target Background

Function(From Uniprot)

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.

Subcellular Location

Virion. Host cytoplasm.

Protein Families

Betacoronavirus nucleocapsid protein family

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