N Recombinant Monoclonal Antibody

Code: CSB-RA33255A1GMY

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50ul100ul

US$210

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Species Reactivity: Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Application: ELISA, WB, GICA

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Target Background

Product Details

Uniprot NO.

P0DTC9

Alternative Names

N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N

Species Reactivity

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Immunogen

Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (CSB-EP3325GMY)

Immunogen Species

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Mouse scFv fusion with human IgG1 Fc

Clone No.

1A6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, GICA

Recommended Dilution

Application	Recommended Dilution
ELISA	1:10000-1:50000
WB	1:500-1:5000
GICA	1:1000-1:10000

Protocols

ELISA Protocol Western Blotting(WB) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli (CSB-EP3325GMY)
SARS-CoV-2 nucleocapsid antibody at 1:1000 (CSB-RA33255A1GMY)
Secondary
Peroxidase-Affinipure Goat Anti-Human IgG Fc Fragment Specific at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 55 kDa
The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N
Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC₅₀ is 1.586 ng/ml.
In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (CSB-RA33255A1GMY) is clean, the detection limit can be as low as 125ng/ml (8.75ng/0.07ml), and the sensitivity is very good.
ELISA: Immobilize various types of SARS proteins at concentration of 2μg/ml on solid substrate, then react with SARS-CoV-2-N Antibody at concentration of 100μg/ml, 10μg/ml and 1μg/ml. It shows the SARS-CoV-2-N Antibody (CSB-RA33255A1GMY) is specific for SARS-CoV-2-N protein, without any cross-reactivity with NL63-CoV, HCoV-OC43, HCoV-229E or HCoV-HKU1.
The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC₅₀ of N Recombinant Antibody.
The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC₅₀ of N Recombinant Antibody.
The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC₅₀ of N Recombinant Antibody.

Description

The SARS-CoV-2 nucleocapsid protein plays a central role in viral replication and assembly, making it one of the most abundant and immunogenic proteins produced during infection. Its high expression levels and structural conservation have established it as a primary target for diagnostic development and fundamental coronavirus research, enabling detection of viral presence and investigation of host-pathogen interactions.

This recombinant monoclonal antibody targeting the SARS-CoV-2 nucleocapsid protein offers the consistency and reliability that demanding research applications require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring your experimental results remain reproducible across studies. The mouse scFv fusion with human IgG1 Fc format provides stable performance while maintaining compatibility with standard secondary detection systems.

Validation data demonstrates exceptional specificity for SARS-CoV-2 nucleocapsid protein without cross-reactivity against related coronavirus proteins including NL63-CoV, HCoV-OC43, HCoV-229E, and HCoV-HKU1, a critical consideration when distinguishing SARS-CoV-2 from endemic human coronaviruses. In Western blot analysis using His-tagged recombinant nucleocapsid protein expressed in E. coli, the antibody detects a band at approximately 55 kDa, slightly higher than the predicted 48 kDa molecular weight, likely reflecting post-translational modifications or anomalous migration patterns common with highly basic proteins like nucleocapsid. Functional ELISA testing reveals strong binding activity with an EC50 of 1.586 ng/ml, while colloidal gold immunochromatography assay validation demonstrates clean background signal with detection sensitivity reaching 125 ng/ml.

This antibody supports researchers developing SARS-CoV-2 diagnostic platforms, studying viral protein biology, or requiring a dependable nucleocapsid detection reagent across ELISA, Western blot, and lateral flow applications.

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Citations

High-Sensitivity Detection of Infectious Disease via Fe3O4@UiO-66-NH2 Combined with a Graphene Field-Effect Transistor M Wang, G Zhou, W Hai, Y Zhang,/,2025

Target Background

Function(From Uniprot)

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.

Subcellular Location

Virion. Host cytoplasm.

Protein Families

Betacoronavirus nucleocapsid protein family

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