N Recombinant Monoclonal Antibody, FITC conjugated

CSB-RA33255C1GMY

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Size:

50ul100ul

US$210

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Species Reactivity: Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

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Target Background

Product Details

Uniprot NO.

P0DTC9

Alternative Names

N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N

Species Reactivity

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Immunogen

Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)

Conjugate

FITC

Clonality

Monoclonal

Isotype

Mouse scFv fusion with human IgG1 Fc

Clone No.

1A6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Description

The creation of the N recombinant monoclonal antibody follows a rigorous process to ensure its quality and specificity. It begins with the isolation of B cells from the spleen of an immunized animal, using the recombinant human SARS-CoV-2 N protein (1-419aa) as the immunogen. The RNA is extracted from these B cells and converted into cDNA through reverse transcription. The N antibody genes are then amplified using specific primers targeting the antibody constant regions and inserted into an expression vector. The human IgG1 Fc is integrated into the vector, downstream of the N antibody gene and the FITC is also inserted into the vector. This recombinant vector is transfected into host cells, allowing for the production of the N recombinant monoclonal antibody. The antibody is harvested from the cell culture supernatant and subjected to purification using affinity chromatography, resulting in a highly purified form. The purified N recombinant monoclonal antibody can be used to detect human SARS-CoV-2 N protein. Through this meticulous production process, a reliable and effective N recombinant monoclonal antibody is obtained, which serves as a valuable tool in various research related to SARS-CoV-2.

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Citations

SARS-CoV-2 infection exacerbates the cellular pathology of Parkinson's disease in human dopaminergic neurons and a mouse model B Lee,/,2024

Target Background

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.

Subcellular Location

Virion. Host cytoplasm.

Protein Families

Betacoronavirus nucleocapsid protein family

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