N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
Species Reactivity
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Immunogen
Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)
Immunogen Species
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Concentration
It differs from different batches. Please contact us to confirm it.
Form
Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Capture: CSB-EAP33255A2C Detection: CSB-EAP33255A2B(HRP) Reagents are sufficient for at least 5 x 96 well plates using recommended protocol.
Tested Applications
S-ELISA
Notes
We recommend using the capture antibody at a concentration of 1ug/ml and the detection antibody at a concentration of 0.42ug/ml. Optimal dilutions should be determined experimentally by the researcher.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
CSB-EAP33255A2 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). An antibody specific for SARS-CoV-2 Nucleoprotein (N) has been pre-coated onto the microwells. The SARS-CoV-2 Nucleoprotein (N) protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody HRP conjugated specific for SARS-CoV-2 Nucleoprotein (N) is added to detect the captured SARS-CoV-2 Nucleoprotein (N) protein. Followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.