RAF1

RAF1, also recognized as MAPK 1 or CRAF, is a protein kinase encoded by the RAF1 gene and part of the RAF family, with widespread expression across various cell types. Acting as a crucial regulator in the MAPK signaling pathway, RAF1 phosphorylates and activates MEK1/2, leading to the activation of ERK1/2 and influencing cell proliferation, differentiation, migration, and survival. RAF1's role in cell cycle control, stress response, and apoptosis makes it a significant factor in various cellular processes. Its dysregulation is linked to numerous diseases, particularly cancer, where RAF1 mutations or overactivation can result in uncontrolled cell proliferation and drug resistance, highlighting its potential as a therapeutic target.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Phospho-RAF1 (S621) Recombinant Monoclonal Antibody(CSB-RA019284A621phHU)

Validated Data

Western Blot
Positive WB detected in Hela whole cell lysate(treated with Calyculin A or EGF)
All lanes Phospho-RAF1 antibody at 1.525μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 73 KDa
Observed band size: 73 KDa

Immunofluorescence staining of HepG2 cells(treated with 50mM Calyculin A for 30min) with CSB-RA019284A621phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

The following RAF1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

RAF1 Antibodies

RAF1 Proteins

RAF1 Antibodies

RAF1 Antibodies for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Code	Product Name	Species Reactivity	Application
CSB-PA553776XA01SVG	RAF1 Antibody	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	ELISA, WB (ensure identification of antigen)

RAF1 Antibodies for Rattus norvegicus (Rat)

Code	Product Name	Species Reactivity	Application
CSB-PA950764	Raf1 Antibody	Rat	ELISA,WB

RAF1 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA019284LA01HU	RAF1 Antibody	Human	ELISA, WB, IHC, IF, IP
CSB-PA242762	RAF1 (Ab-259) Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA291059	Phospho-RAF1 (Ser259) Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA151308	Phospho-RAF1 (Ser338) Antibody	Human,Mouse	ELISA,WB
CSB-PA209480	RAF1 (Ab-338) Antibody	Human,Mouse	ELISA,WB,IHC
CSB-PA445340	Phospho-RAF1 (Ser621) Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA580751	Phospho-RAF1 (Tyr341) Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA299750	Phospho-RAF1 (Thr269) Antibody	Human,Mouse	ELISA,IHC
CSB-PA148049	Phospho-RAF1 (Ser642) Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA948259	Phospho-RAF1 (Ser301) Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA000615	Phospho-RAF1 (S259) Antibody	Human,Mouse,Rat,Monkey	WB, IHC, ELISA
CSB-PA000660	Phospho-RAF1 (S338) Antibody	Human,Mouse,Rat	WB, ELISA
CSB-PA003921	RAF1 Antibody	Human,Mouse,Rat	WB, IHC, IF, ELISA
CSB-PA003922	RAF1 Antibody	Human,Mouse,Rat,Monkey	WB, IF, ELISA
CSB-PA003923	RAF1 Antibody	Human,Mouse,Rat	WB, ELISA
CSB-PA003926	RAF1 Antibody	Human,Mouse,Rat	WB, IHC, ELISA
CSB-PA040148	Phospho-RAF1 (S43) Antibody	Human,Mouse,Rat	WB, ELISA
CSB-PA040149	Phospho-RAF1 (S301) Antibody	Human,Mouse,Rat	WB, ELISA
CSB-PA040150	Phospho-RAF1 (S642) Antibody	Human,Mouse,Rat	WB, ELISA
CSB-PA040151	Phospho-RAF1 (Y341) Antibody	Human,Mouse,Rat	WB, IHC, ELISA
CSB-PA040152	Phospho-RAF1 (S296) Antibody	Human,Mouse,Rat	WB, IHC, ELISA
CSB-PA040153	Phospho-RAF1 (S621) Antibody	Human,Mouse,Rat,Monkey	WB, IHC, IF, ELISA
CSB-PA040154	Phospho-RAF1 (T269) Antibody	Human,Mouse,Rat	IHC, ELISA
CSB-PA040155	Phospho-RAF1 (S289) Antibody	Human,Mouse,Rat	IHC, ELISA
CSB-PA040156	RAF1 Antibody	Human,Mouse,Rat	WB, ELISA
CSB-PA040157	RAF1 Antibody	Human,Mouse,Rat	WB, IHC, ELISA
CSB-PA040158	RAF1 Antibody	Human,Mouse,Rat	WB, IHC, ELISA
CSB-PA040160	RAF1 Antibody	Human,Mouse,Rat	IHC, ELISA
CSB-PA080220	RAF1 Antibody	Human	WB, IHC
CSB-PA010547	RAF1 Antibody	Human,Mouse,Rat	WB, ELISA
CSB-PA694669	RAF1 Antibody	Human,Mouse,Rat	ELISA,IHC
CSB-RA019284A621phHU	Phospho-RAF1 (S621) Recombinant Monoclonal Antibody	Human	ELISA, WB, IF
CSB-RA019284A259phHU	Phospho-RAF1 (S259) Recombinant Monoclonal Antibody	Human	ELISA, WB, IHC, IF
CSB-RA019284A43phHU	Phospho-RAF1 (S43) Recombinant Monoclonal Antibody	Human	ELISA, IF

RAF1 Antibodies for Zea mays (Maize)

Code	Product Name	Species Reactivity	Application
CSB-PA184121XA01ZAX	RAF1 Antibody	Zea mays (Maize)	ELISA, WB (ensure identification of antigen)

RAF1 Antibodies for Oryza sativa subsp. japonica (Rice)

Code	Product Name	Species Reactivity	Application
CSB-PA269806XA01OFG	RAF1 Antibody	Oryza sativa subsp. japonica (Rice)	ELISA, WB (ensure identification of antigen)

RAF1 Antibodies for Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Code	Product Name	Species Reactivity	Application
CSB-PA524907XA01SXV	raf1 Antibody	Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)	ELISA, WB (ensure identification of antigen)

RAF1 Proteins

RAF1 Proteins for Gallus gallus (Chicken)

Code	Product Name	Source
CSB-YP019284CH CSB-EP019284CH CSB-BP019284CH CSB-MP019284CH CSB-EP019284CH-B	Recombinant Chicken RAF proto-oncogene serine/threonine-protein kinase (RAF1), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

RAF1 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP019284HU CSB-EP019284HU CSB-BP019284HU CSB-MP019284HU CSB-EP019284HU-B	Recombinant Human RAF proto-oncogene serine/threonine-protein kinase (RAF1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP019284HU	Recombinant Human RAF proto-oncogene serine/threonine-protein kinase (RAF1)	E.coli
CSB-EP019284HUc7	Recombinant Human RAF proto-oncogene serine/threonine-protein kinase (RAF1)	E.coli

RAF1 Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP019284RA CSB-EP019284RA CSB-BP019284RA CSB-MP019284RA CSB-EP019284RA-B	Recombinant Rat RAF proto-oncogene serine/threonine-protein kinase (Raf1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

RAF1 Proteins for Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)

Code	Product Name	Source
CSB-YP722278PYX CSB-EP722278PYX CSB-BP722278PYX CSB-MP722278PYX CSB-EP722278PYX-B	Recombinant Pongo abelii RAF proto-oncogene serine/threonine-protein kinase (RAF1), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

RAF1 Proteins for Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Code	Product Name	Source
CSB-YP524907SXV CSB-EP524907SXV CSB-BP524907SXV CSB-MP524907SXV CSB-EP524907SXV-B	Recombinant Schizosaccharomyces pombe Rik1-associated factor 1 (raf1), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

RAF1 Proteins for Bos taurus (Bovine)

Code	Product Name	Source
CSB-YP019284BO CSB-EP019284BO CSB-BP019284BO CSB-MP019284BO CSB-EP019284BO-B	Recombinant Bovine RAF proto-oncogene serine/threonine-protein kinase (RAF1), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

RAF1 Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP859572MO CSB-EP859572MO CSB-BP859572MO CSB-MP859572MO CSB-EP859572MO-B	Recombinant Mouse RAF proto-oncogene serine/threonine-protein kinase (Raf1)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

RAF1 Proteins for Xenopus laevis (African clawed frog)

Code	Product Name	Source
CSB-YP019284XBE CSB-EP019284XBE CSB-BP019284XBE CSB-MP019284XBE CSB-EP019284XBE-B	Recombinant Xenopus laevis RAF proto-oncogene serine/threonine-protein kinase (raf1), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

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