Raf1 Antibody

Code: CSB-PA950764

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Size:

100μl

US$297

Quantity:

Species Reactivity: Rat

Raised in: Rabbit

Application: ELISA,WB

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Target Background

Product Details

Uniprot NO.

P11345

Target Names

Raf1

Alternative Names

Raf1 antibody; Raf antibody; RAF proto-oncogene serine/threonine-protein kinase antibody; EC 2.7.11.1 antibody; Proto-oncogene c-RAF antibody; cRaf antibody; Raf-1 antibody

Raised in

Rabbit

Species Reactivity

Rat

Immunogen

Peptide sequence around aa. 641-645(T-S-P-R-L ) derived from Rat Raf-1.

Immunogen Species

Rattus norvegicus (Rat)

Clonality

Polyclonal

Purification Method

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide

Concentration

It differs from different batches. Please contact us to confirm it.

Form

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA,WB

Protocols

ELISA Protocol Western Blotting(WB) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western blot analysis of extract from mouse brain tissue and C6 cells using Raf-1 Antibody

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Target Background

Function(From Uniprot)

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation. Phosphorylates TNNT2/cardiac muscle troponin T.

Gene References into Functions

Wild-type PKGII phosphorylated Raf-1 at Ser-43 in a cGMP-dependent manner, but a PKGII D412A/R415A mutant, which has a low affinity for cGMP, did not. PMID:28057484
Raf-1 kinase contributes to cerebral vasospasm and early brain injury after subarachnoid hemorrhage by enhancing the activation of the Raf-1/ERK1/2 and Raf-1/NF-kappaB signaling pathways. PMID:25367879
sorafenib exhibits a regulatory role on the occurrence of LVH following AB in rats by blocking the rise in growth factors PDGF-BB and TGFbeta1, activation of the corresponding c-Raf-ERK1/2 signaling pathway PMID:24885948
Our results demonstrate that XIAP plays a new role as a negative regulator of neurotrophin-induced neurite outgrowth and neuronal differentiation in developing neurons. PMID:23928917
Induction of streptozotocin diabetes resulted in decreased level of GLP-1R and increased levels of IRS-1 and Raf-1 in the liver. PMID:22983684
Data show that a dimeric Raf kinase inhibitor protein (RKIP) mutant displayed a higher affinity to G protein-coupled receptor kinase (GRK) 2, but a lower affinity to Raf1. PMID:22610096
Protein arginine methyltransferase 5 regulates ERK1/2 signal transduction amplitude and cell fate through CRAF. PMID:21917714
RAF1 mutants caused hypertrophy signaled via nuclear factor of activated T cells PMID:21440552
These results indicate the initial robust activation of ERK phosphorylates Raf-1 at Ser289/296/301, resulting in Raf-1inhibition and then prompt inactivation of ERK following sublethal preconditioning ischemia. PMID:20953701
Raf-1 played a significant role in sustained morphine-mediated paradoxical pain sensitization and antinociceptive tolerance in vivo. PMID:20718739
Raf-1 kinase plays an important role in the regulation of vascular contractility through regulation of calcium sensitization. PMID:20110729
the role of Raf-1 in v-Ha-ras transformation PMID:11933072
Affinity with Raf is sufficient for Ras to efficiently induce rat mammary carcinomas PMID:12538354
Raf-1 has a role in modulating integrin-stimulated ERK activation when bound to CAMKII PMID:12954639
Changes in flexibility upon protein-protein complex formation of H-Ras & the Ras-binding domain of C-Raf1 have been investigated using the molecular framework approach FIRST and molecular dynamics simulations of in total approximately 35 ns length. PMID:15211515
selective modulation of c-Raf-1 but not B-Raf activation by RKIP can limit the dynamic range of the MAPK signaling response to growth factors and may play a critical role in growth and development PMID:15886202
aldosterone seems to exert mainly MR-induced effects that stimulate c-Raf, MEK1/2, MAPK1/2, the activities of CDK2 and CDK4, and the cell-cycle progression in mesangial cells PMID:15975997
c-raf.1 expression was located in the late basophilic lesions associated with hepatocellular carcinoma. PMID:17554210
Our results suggest that ROS production mediates the biphasic activation of ERK through different signaling cascades following post-ischemic reperfusion. Mediation of these signaling pathways involves changes in Raf-1 phosphorylation at different sites. PMID:18243549
AhR is a master regulator of c-raf and propose cross-talk between AhR and the mitogen-activated protein kinase signaling pathway. PMID:18708364
These studies identify Raf-dependent cTnT-Thr(206) phosphorylation as a novel mechanism that would link growth factor-dependent signaling pathways to dynamic changes in cardiac contractile function. PMID:19381846