raf1 Antibody

Code: CSB-PA524907XA01SXV

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Size:

0.1ml

0.1ml1ml

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Species Reactivity: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Raised in: Rabbit

Application: ELISA, WB (ensure identification of antigen)

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Target Background

Product Details

Uniprot NO.

O74910

Target Names

raf1

Alternative Names

raf1 antibody; clr8 antibody; cmc1 antibody; dos1 antibody; SPCC613.12c antibody; Rik1-associated factor 1 antibody; Cryptic loci regulator 8 antibody; De-localization of swi6 protein 1 antibody

Raised in

Rabbit

Species Reactivity

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Immunogen

Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) raf1 protein

Immunogen Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB (ensure identification of antigen)

Lead Time

Made-to-order (14-16 weeks)
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

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Target Background

Function(From Uniprot)

Component of the Clr4 methyltransferase complex (ClrC) which contributes to the establishment of heterochromatin by specifically methylating histone H3 to form H3K9me. ClrC preferentially ubiquitylates H3K14 and ClrC-mediated H3 ubiquitination promotes clr4 methyltransferase activity for the methylation of H3K9. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting swi6/HP1 to methylated histones which leads to transcriptional silencing within centromeric heterochromatin, telomeric regions and at the silent mating-type loci. Has a role in both mitotic and meiotic chromosome segregation.

Gene References into Functions

Disruption of Dos1, Dos2, or Cdc20 results in dissociation of CENP-A from centromeres and mislocalization of the protein to noncentromeric sites during S phase. PMID:23267073
Thus the association of a DCAF, Raf1, with its adaptor, Rik1, is required for histone methylation and to allow RNAi to signal to chromatin PMID:22319459
Dos1 acts in the RNAi-dependent heterochromatin pathway. It is required for silencing of heterochromatin regions, for H3K9me association with centromeric repeats, for production of siRNAs, and for Swi6 localization. It directly interacts with Rik1. [dos1] PMID:16040243

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Subcellular Location

Cytoplasm. Nucleus. Chromosome.

Database Links

KEGG: spo:SPCC613.12c

STRING: 4896.SPCC613.12c.1

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