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Recombinant Schizosaccharomyces pombe Rik1-associated factor 1 (raf1), partial

Recombinant Schizosaccharomyces pombe Rik1-associated factor 1 (raf1), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

raf1

Uniprot NO.

O74910

Alternative Names

raf1; clr8; cmc1; dos1; SPCC613.12c; Rik1-associated factor 1; Cryptic loci regulator 8; De-localization of swi6 protein 1

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Source

Yeast

Expression Region

1-638

Target Protein Sequence

MTNSSPRVKR RTDTQYLHSV SSKLPKVDFD TNNEEFFEEE FEIYDPFYRA ELPCPKPSLS ISKHSIAKVP SNVNKRLELQ LLLTSGTFLP NSRPYLSERV RKHTHLLSNS ITGDDKPSLI HVDFTPEECF ILQEAKLKFG PVNSVQFNDA YSTHISPKLP GRAYEDCQKF EIDNPSLSPV DKHGAIILRT YKKNKKLLPD YLKSFYNAGS SYFQREQVHQ LMDGESVFFL KPWKHFNETS GDTVCVAYNP LCEKFALGST AQDGAYNRLG NLWIGDFHSE TIQSLESHYK LNQVGEKEYS TISDLCFSKG NLFLYTGAFD NAVKVWDMEG NLCGIFNAPT DYIHKLALSD DDLLAVACKN GYGYLLSTDN STGEILTSAN LIYPEALEKG YSASLIEFSN FLGRSSDKVI IGYDSFHTSN NRGCLALFDA STASFVQKFN TADEAFTSLY MHPSQVGFVA SSNTLSNGRV YYLDTRMYKV CLNFTTTQKD INHATISNSG ILVTSSGTDN QTFVWDSRKP DKPLSLLKHG KTKMIHFDGA NEEEVDAGIN MAQWQPKGNL FVTGGSDGIV KVWDLRLNNP FIQNFTEMNS AITYGGFSED ASKLTVCCVG GDVNMYSLGN DNGNKFGEFR IIENRLLT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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Target Background

Function(From Uniprot)

Component of the Clr4 methyltransferase complex (ClrC) which contributes to the establishment of heterochromatin by specifically methylating histone H3 to form H3K9me. ClrC preferentially ubiquitylates H3K14 and ClrC-mediated H3 ubiquitination promotes clr4 methyltransferase activity for the methylation of H3K9. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting swi6/HP1 to methylated histones which leads to transcriptional silencing within centromeric heterochromatin, telomeric regions and at the silent mating-type loci. Has a role in both mitotic and meiotic chromosome segregation.

Gene References into Functions

Disruption of Dos1, Dos2, or Cdc20 results in dissociation of CENP-A from centromeres and mislocalization of the protein to noncentromeric sites during S phase. PMID:23267073
Thus the association of a DCAF, Raf1, with its adaptor, Rik1, is required for histone methylation and to allow RNAi to signal to chromatin PMID:22319459
Dos1 acts in the RNAi-dependent heterochromatin pathway. It is required for silencing of heterochromatin regions, for H3K9me association with centromeric repeats, for production of siRNAs, and for Swi6 localization. It directly interacts with Rik1. [dos1] PMID:16040243

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Subcellular Location

Cytoplasm. Nucleus. Chromosome.

Database Links

KEGG: spo:SPCC613.12c

STRING: 4896.SPCC613.12c.1

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