LY6G6D Recombinant Monoclonal Antibody

Code: CSB-RA013246MA4HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human, Mouse, Rat, Macaca fascicularis

Application: ELISA, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

O95868

Target Names

LY6G6D

Alternative Names

Lymphocyte antigen 6 complex locus protein G6d; Protein Ly6-D; Megakaryocyte-enhanced gene transcript 1 protein; LY6G6D; C6orf23; G6D; MEGT1; NG25

Species Reactivity

Human, Mouse, Rat, Macaca fascicularis

Immunogen

Recombinant Human LY6G6D protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

mIgG1

Clone No.

9H7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

The Binding Activity of Macaca fascicularis LY6G6D with Anti-LY6G6D Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis LY6G6D (CSB-YP4607MOV) at 2 μg/mL can bind Anti-LY6G6D recombinant antibody. The EC₅₀ is 19.33-25.47 ng/mL.
The Binding Activity of Mouse Ly6g6d with Anti-LY6G6D Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Ly6g6d (CSB-YP4973MO) at 2 μg/mL can bind Anti-LY6G6D recombinant antibody. The EC₅₀ is 5.347-7.161 ng/mL.
The Binding Activity of Human LY6G6D with Anti-LY6G6D Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human LY6G6D (CSB-YP013246HU) at 2 μg/mL can bind Anti-LY6G6D recombinant antibody. The EC₅₀ is 10.57-13.27 ng/mL.
The Binding Activity of Rat Ly6g6d with Anti-LY6G6D Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Rat Ly6g6d (CSB-YP750592RA) at 2 μg/mL can bind Anti-LY6G6D recombinant antibody. The EC₅₀ is 5.825-7.131 ng/mL.
Untransfected HEK-293T cells (green line) and transfected Human LY6G6D HEK-293T stable cells (red line) were stained with anti-LY6G6D antibody (CSB-RA013246MA4MO) (2µg/1*10⁶cells), washed and then followed by FITC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry.
Untransfected CT26 cells (green line) and transfected Human LY6G6D CT26 stable cells (red line) were stained with anti-LY6G6D antibody (CSB-RA013246MA4MO) (2µg/1*10⁶cells), washed and then followed by FITC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry.
The purity of LY6G6D was greater than 95% as determined by SEC-HPLC

Description

LY6G6D belongs to the lymphocyte antigen 6 superfamily, a group of GPI-anchored cell surface proteins that play important roles in immune cell development and function. Also known as MEGT1 (megakaryocyte-enhanced gene transcript 1 protein), LY6G6D has garnered attention in immunology research for its involvement in immune cell signaling and its potential as a marker in various disease contexts.

This recombinant monoclonal antibody (clone 9H7) offers the consistency and reliability that demanding research protocols require. Because it is produced from a defined sequence rather than traditional hybridoma methods, you can expect uniform performance across different lots, eliminating the variability that can compromise longitudinal studies or multi-site collaborations. The mouse IgG1 isotype and affinity-chromatography purification ensure high purity, confirmed at greater than 95% by SEC-HPLC analysis.

Functional validation demonstrates robust binding activity across multiple species, providing genuine cross-species reactivity for translational research workflows. ELISA testing shows strong binding to human, mouse, rat, and cynomolgus macaque LY6G6D proteins, with EC50 values in the low nanogram per milliliter range across all species tested. Flow cytometry validation further confirms specificity, with clear discrimination between untransfected control cells and LY6G6D-transfected stable cell lines in both HEK-293T and CT26 backgrounds. For flow cytometry applications, dilutions of 1:50 to 1:200 provide optimal results.

Whether you are investigating immune cell populations, exploring LY6G6D as a potential biomarker, or conducting comparative studies across preclinical models, this antibody delivers the specificity and cross-species utility needed to advance your immunology research.