LY6G6D Recombinant Monoclonal Antibody

Code: CSB-RA013246MA2HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human, Mouse, Rat, Macaca fascicularis

Application: ELISA, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

O95868

Target Names

LY6G6D

Alternative Names

Lymphocyte antigen 6 complex locus protein G6d; Protein Ly6-D; Megakaryocyte-enhanced gene transcript 1 protein; LY6G6D; C6orf23; G6D; MEGT1; NG25

Species Reactivity

Human, Mouse, Rat, Macaca fascicularis

Immunogen

Recombinant Human LY6G6D protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

mIgG2b

Clone No.

2D6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

The Binding Activity of Rat Ly6g6d with Anti-LY6G6D Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Rat Ly6g6d (CSB-YP750592RA) at 5 μg/mL can bind Anti-LY6G6D recombinant antibody. The EC₅₀ is 4.245-5.843 μg/mL.
The Binding Activity of Human LY6G6D with Anti-LY6G6D Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human LY6G6D (CSB-YP013246HU) at 2 μg/mL can bind Anti-LY6G6D recombinant antibody. The EC₅₀ is 1.840-2.204 ng/mL.
The Binding Activity of Mouse Ly6g6d with Anti-LY6G6D Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Ly6g6d (CSB-YP4973MO) at 2 μg/mL can bind Anti-LY6G6D recombinant antibody. The EC₅₀ is 1.159-2.305 μg/mL.
The Binding Activity of Macaca fascicularis LY6G6D with Anti-LY6G6D Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis LY6G6D (CSB-YP4607MOV) at 2 μg/mL can bind Anti-LY6G6D recombinant antibody. The EC₅₀ is 16.16-25.29 ng/mL.
Untransfected HEK293T cells (green line) and transfected Human LY6G6D HEK293T stable cells (red line) were stained with anti-LY6G6D antibody (2µg/1*10⁶cells), washed and then followed by FITC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry.
Untransfected CT26 cells surface (green line) and transfected Human LY6G6D CT26 stable cells surface (red line) were stained with anti-LY6G6D antibody (2µg/1*10⁶cells), washed and then followed by FITC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry.

Description

LY6G6D belongs to the lymphocyte antigen 6 superfamily, a group of GPI-anchored cell surface proteins implicated in immune cell signaling and differentiation. Also known as MEGT1 (megakaryocyte-enhanced gene transcript 1 protein), LY6G6D has garnered attention for its potential roles in hematopoietic cell biology and as an emerging target in oncology research, where its expression patterns may serve as markers for specific cell populations or disease states.

This recombinant monoclonal antibody, clone 2D6, offers the reproducibility and sequence-defined consistency that demanding experimental workflows require. Produced in mouse as an IgG2b isotype and purified by affinity chromatography, this antibody eliminates the lot-to-lot variability inherent in traditional hybridoma-derived reagents, ensuring your results remain comparable across extended studies.

Functional ELISA validation demonstrates robust binding activity across multiple species, with confirmed reactivity to human, mouse, rat, and Macaca fascicularis LY6G6D proteins. The antibody shows particularly strong affinity for human LY6G6D, with EC50 values in the low nanogram per milliliter range. Flow cytometry validation further confirms performance in cell-based applications, with clear discrimination between untransfected and LY6G6D-transfected cells demonstrated in both HEK293T and CT26 cell lines at 2 μg per million cells.

This cross-species reactivity makes the antibody especially valuable for translational research programs requiring validation across preclinical models and human samples. Whether investigating LY6G6D in immune cell characterization, megakaryocyte biology, or tumor immunology contexts, this antibody provides a reliable tool for flow cytometry and ELISA-based detection strategies.