GPC3

GPC3 encodes glypican-3, a cell surface heparan sulfate proteoglycan. It modulates growth factor signaling by binding molecules like Wnt and FGF, regulating cell proliferation and differentiation. Overexpression in hepatocellular carcinoma (HCC) promotes tumor growth via Wnt/β-catenin pathway activation, while loss-of-function mutations cause Simpson-Golabi-Behmel syndrome, an overgrowth disorder.
As an HCC-specific antigen, GPC3 is a therapeutic target. Monoclonal antibodies (e.g., codrituzumab) and CAR-T cell therapies are under clinical evaluation. These agents aim to block GPC3-mediated signaling or induce immune-mediated tumor cell killing, showing promise in early-phase trials for advanced HCC.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized human GPC3 at 5 μg/ml can bind Anti-GPC3 recombinant antibody, the EC₅₀ is 35.19-52.30 ng/ml.

GPC3 Recombinant Monoclonal Antibody(CSB-RA009705A1HU)

Validated Data

The Binding Activity of Human GPC3 with Anti-GPC3 recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human GPC3 (CSB-MP009705HU(M)) at 2 μg/mL can bind Anti- GPC3 recombinant Antibody, the EC₅₀ is 2.783-3.653 ng/mL.

GPC3 Monoclonal Antibody(CSB-MA009705A0m)

Validated Data

Western Blot
Positive WB detected in: GPC3 antibody at 1:1000
Lane 1: Jurkat whole cell lysate
Lane 2: Mouse liver tissue
Secondary
Goat polyclonal to Mouse IgG at 1/20000 dilution
Predicted band size: 65-70KDa
Observed band size: 65-70 KDa
Exposure time: 5min

Overlay Peak curve showing Jurkat cells stained with CSB-MA009705A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Human Glypican-3,GPC-3 ELISA Kit(CSB-E11333h)

Validated Data

Code: CSB-E11333h

Size: 96T,5×96T,10×96T

Sensitivity: 0.156 ng/mL

Detection Range: 0.625 ng/mL-40 ng/mL

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

The following GPC3 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

GPC3 Antibodies

GPC3 Proteins

GPC3 ELISA Kits

GPC3 Antibodies

GPC3 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA009705GA01HU	GPC3 Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA005135	GPC3 Antibody	Human,Mouse,Rat	WB, ELISA
CSB-PA12749A0Rb	GPC3 Antibody	Human	ELISA, IF
CSB-PA12749B0Rb	GPC3 Antibody, HRP conjugated	Human	ELISA
CSB-PA12749C0Rb	GPC3 Antibody, FITC conjugated	Human
CSB-PA12749D0Rb	GPC3 Antibody, Biotin conjugated	Human	ELISA
CSB-MA009705A0m	GPC3 Monoclonal Antibody	Human	ELISA, WB, FC
CSB-RA009705A0HU	GPC3 Recombinant Monoclonal Antibody	Human	ELISA, WB, IF
CSB-RA009705A1HU	GPC3 Recombinant Monoclonal Antibody	Human	ELISA
CSB-RA009705MA1HU	GPC3 Recombinant Monoclonal Antibody	Human	ELISA, FC
CSB-RA009705MB1HU	GPC3 (Codrituzumab Biosimilar) Recombinant Monoclonal Antibody	Human
CSB-RA009705MB2HU	GPC3 (MDX-1414 Biosimilar) Recombinant Monoclonal Antibody	Human

GPC3 Proteins

GPC3 Proteins for Pan troglodytes (Chimpanzee)

Code	Product Name	Source
CSB-YP009705EQV CSB-EP009705EQV CSB-BP009705EQV CSB-MP009705EQV CSB-EP009705EQV-B	Recombinant Pan troglodytes Glypican-3 (GPC3), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-MP009705EQV	Recombinant Pan troglodytes Glypican-3 (GPC3)	Mammalian cell

GPC3 Proteins for Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Code	Product Name	Source
CSB-MP7605MOV	Recombinant Macaca fascicularis glypican-3 (GPC3), partial (Active)	Mammalian cell

GPC3 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP009705HU CSB-EP009705HU CSB-BP009705HU CSB-MP009705HU CSB-EP009705HU-B	Recombinant Human Glypican-3 (GPC3), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-AP005371HU	Recombinant Human Glypican-3 (GPC3), partial (Active)	Mammalian cell
CSB-MP009705HU(M)	Recombinant Human Glypican-3 (GPC3) (G537R), partial (Active)	Mammalian cell
CSB-MP009705HU(M2)	Recombinant Human Glypican-3 (GPC3)(S359F), partial (Active)	Mammalian cell
CSB-MP009705HUc7	Recombinant Human Glypican-3 (GPC3), partial (Active)	Mammalian cell
CSB-MP009705HU1	Recombinant Human Glypican-3 (GPC3), partial (Active)	Mammalian cell
CSB-MP009705HU4	Recombinant Human Glypican-3 (GPC3), partial	Mammalian cell

GPC3 Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP009705RA	Recombinant Rat Glypican-3 (Gpc3), partial	Yeast
CSB-YP009705RA CSB-EP009705RA CSB-BP009705RA CSB-MP009705RA CSB-EP009705RA-B	Recombinant Rat Glypican-3 (Gpc3), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

GPC3 Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP804039MO CSB-EP804039MO CSB-BP804039MO CSB-MP804039MO CSB-EP804039MO-B	Recombinant Mouse Glypican-3 (Gpc3), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-MP804039MO-B	Recombinant Mouse Glypican-3 (Gpc3), partial, Biotinylated	Mammalian cell
CSB-EP804039MO	Recombinant Mouse Glypican-3 (Gpc3)	E.coli