Abbreviation
Recombinant Human GPC3 protein (S359F), partial (Active)
Purity
Greater than 90% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Loaded Anti-Human GPC3 Antibody (CSB-RA009705A1HU) on 96-Flat plate, can bind Human GPC3(S359F), with an affinity constant of 6.32 nM as determined in BLI assay (Gator Prime).
Species
Homo sapiens (Human)
Expression Region
25-559aa(S359F)
Target Protein Sequence
QPPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQLMNPGLPDSALDINECLRGARRDLKVFGNFPKLIMTQVSKSLQVTRIFLQALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNAGKLTTTIGKLCAHSQQRQYRFAYYPEDLFIDKKVLKVAHVEHEETLSSRRRELIQKLKSFISFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSMPKGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPGNSQQATPKDNEISTFHNLGNVH
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
-
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
-
Activity
Loaded Anti-Human GPC3 Antibody (CSB-RA009705A1HU) on 96-Flat plate, can bind Human GPC3(S359F), with an affinity constant of 6.32 nM as determined in BLI assay (Gator Prime).
Description
Glypican-3 serves as a critical oncofetal antigen overexpressed in hepatocellular carcinoma and other malignancies, making it a high-priority target for cancer immunotherapy development and diagnostic antibody validation. This mammalian-expressed construct spanning residues 25–559 with an S359F mutation preserves native glycosylation patterns essential for authentic receptor interactions, and demonstrates a binding affinity of 6.32 nM to anti-GPC3 antibodies in biolayer interferometry assays—a quantitative benchmark that supports its use as a coating antigen for antibody validation, integrin-binding assays, and receptor interaction studies in cancer invasion models. The low endotoxin level of less than 1.0 EU/μg combined with greater than 90% purity meets the quality thresholds commonly required for cell-based functional assays, including 3D culture systems investigating tumor microenvironment interactions and matrix remodeling experiments. Researchers studying GPC3-mediated signaling in hepatocellular carcinoma or developing therapeutic antibodies will find this construct provides a suitable basis for mechanistic studies and immunoassay standardization.
-SDS.jpg)
-AC1.jpg)
