Abbreviation
Recombinant Rhesus macaque TNF protein, partial (Active)
Purity
>95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of >2.0x107 IU/mg in the presence of actinomycin D.
Alternative Names
TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
Species
Macaca mulatta (Rhesus macaque)
Expression Region
77-233aa
Target Protein Sequence
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
0.2 μm filtered PBS, pH 7.4, 5 % trehalose ,lyophilized
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
TNF-α is a master regulator of NF-κB and MAPK signaling cascades, making precise functional characterization in non-human primate models essential for translational oncology and inflammation research. This tag-free recombinant rhesus macaque TNF protein (aa 77–233) demonstrates exceptional signaling potency, with an ED50 below 0.05 ng/ml in the murine L929 cytotoxicity assay—corresponding to a specific activity exceeding 2.0 × 10⁷ IU/mg—providing a reliable basis for NF-κB pathway activation studies, apoptosis assays, and dose-response benchmarking as a reference standard in cytokine detection platforms. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that confound immune cell activation and differentiation experiments, while purity greater than 95% by SDS-PAGE supports use in antibody development workflows including immunization, ELISA coating, and positive control applications. The mature sequence fidelity of this E. coli-expressed protein aligns with standards expected in preclinical tumor biology models requiring species-matched cytokine stimulation.
