Monkey Tumor necrosis factor α,TNF-α ELISA KIT

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Code: CSB-E10039Mo
Size:
96T,5×96T,10×96T
96T,5×96T,10×96T
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Quantity:
Sample Types: serum, plasma, tissue homogenates
Detect Range: 4.69 pg/mL-300 pg/mL
Sensitivity: 2.345 pg/mL
Trial Size:
24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >>
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Product Details

Alternative Names
TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
Abbreviation
TNF
Uniprot NO.
Species
Monkey
Sample Type
serum, plasma, tissue homogenates
Detection Range
4.69 pg/mL-300 pg/mL
Sensitivity
2.345 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Measurement
Sandwich
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Precision

Typical Data

Troubleshooting and FAQs
Description

This Monkey TNF ELISA Kit was designed for the quantitative measurement of Monkey TNF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

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Sample species : a:1:{i:0;a:2:{i:0;s:6:"Monkey";i:1;s:0:"";}}

Sample type: a:1:{i:0;a:2:{i:0;s:24:"Cell culture supernatant";i:1;s:0:"";}}

Sample dilution: a:1:{i:0;a:2:{i:0;s:5:"Other";i:1;s:12:"1:3,1:5 1:10";}}

Review: CSB-E10039Mo was used to detect the culture supernatant of cynomolgus monkey cells. The OD value of standard curve ranged from 3.1274 to 0.2045, and the OD value of sample ranged from 1.112 to 0.109.

By Anonymous

Target Background

Function(From Uniprot)
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Gene References into Functions
  1. These interactions result in the induction of the TNF signaling pathway, activation of apoptosis, and DNA-damage stress response. PMID:24743303
  2. Findings showed that both mucosal compartments harbor similar percentages of memory CD4(+) T cells and displayed comparable cytokine TNF-alpha responses to mitogenic stimulations prior to infection. PMID:24610016
  3. Primary role for IL-1beta and TNF-alpha in the triggering of preterm labor associated with inflammation or infection. PMID:17132473

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Subcellular Location
Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
Protein Families
Tumor necrosis factor family
Database Links

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