Abbreviation
Recombinant Human IFNA2 protein (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.0x108 IU/mg.
Alternative Names
IFN-alpha-2, LeIF A
Species
Homo sapiens (Human)
Expression Region
24-188aa
Target Protein Sequence
CDLPQTHSLG SRRTLMLLAQ MRKISLFSCL KDRHDFGFPQ EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES LRSKE
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
Interferon alpha-2 is a pleiotropic cytokine central to antiviral defense and immunomodulation, and this tag-free recombinant form delivers a specific activity of no less than 1.0 × 10⁸ IU/mg in anti-viral assays, confirming robust signaling potency suitable for JAK/STAT pathway activation studies and cell-based functional assays including PBMC stimulation and tumor cell antiproliferative experiments. The full-length mature sequence (aa 24–188) expressed in yeast ensures proper folding without prokaryotic contaminants, while the tag-free format eliminates steric interference that could confound receptor-binding kinetics in antibody development workflows or when used as a reference standard in interferon detection assays. Endotoxin levels below 1.0 EU/μg prevent LPS-driven artifacts in sensitive immune cell activation and differentiation experiments, and purity exceeding 97% by SDS-PAGE supports use in in vivo models of infection or tumor biology where dose accuracy depends on minimal protein contaminants.
