Abbreviation
Recombinant Human IFNA2 protein (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized Human IFNA2 at 2 μg/mL can bind Human IFNAR2(CSB-MP011047HU). The EC50 is 154.2-191.9 ng/mL.②Measured by its binding ability in a functional ELISA. Immobilized Human IFNA2 at 2 μg/mL can bind Anti-IFNA2 recombinant antibody(CSB-RA365593MA1HU). The EC50 is 2.366-2.818 ng/mL.
Alternative Names
IFN-alpha-2; Interferon alpha-A (LeIF A 1)
Species
Homo sapiens (Human)
Expression Region
24-188aa
Target Protein Sequence
CDLPQTHSLGSRRTLMLLAQMRRISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEMIQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAVRKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human IFNA2 at 2 μg/ml can bind Human IFNAR2(CSB-MP011047HU). The EC50 is 154.2-191.9 ng/mL.
-
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human IFNA2 at 2 μg/ml can bind Anti-IFNA2 recombinant antibody(CSB-RA360706MA2HU). The EC50 is 2.366-2.818 ng/mL.
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Description
Interferon alpha-2 plays a central role in antiviral immunity and tumor surveillance by activating JAK/STAT signaling cascades, yet many commercial preparations suffer from endotoxin contamination that confounds immune cell readouts. This full-length mature protein (aa 24–188) demonstrates robust receptor engagement, binding human IFNAR2 with an EC50 of 154.2–191.9 ng/mL in functional ELISA, a quantitative measure that supports its use in cell-based proliferation assays with TF-1 cells or primary PBMCs, JAK/STAT phosphorylation studies, and apoptosis induction experiments where dose-dependent signaling is critical. The endotoxin level below 1.0 EU/μg meets the quality thresholds commonly required for immune cell activation assays, ensuring that observed effects reflect interferon-mediated biology rather than LPS-driven artifacts. Antibody binding with an EC50 of 2.366–2.818 ng/mL confirms epitope accessibility, making this protein appropriate for antibody development workflows and as a positive control in ELISA or Western blot validation, while the >95% purity by SDS-PAGE provides a suitable basis for in vivo tumor models and cytokine detection assay standardization.
