TNFRSF1B Recombinant Monoclonal Antibody

Code: CSB-RA159920A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, WB, IF, FC

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Product Details
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Target Background

Product Details

Uniprot NO.

P20333

Target Names

TNFRSF1B

Alternative Names

CD120b antibody; p75 antibody; p75 TNF receptor antibody; p75TNFR antibody; p80 TNF alpha receptor antibody; p80 TNF-alpha receptor antibody; Soluble TNFR1B variant 1 antibody; TBP-2 antibody; TBPII antibody; TNF R II antibody; TNF R2 antibody; TNF R75 antibody; TNF-R2 antibody; TNF-RII antibody; TNFBR antibody; TNFR-II antibody; TNFR1B antibody; TNFR2 antibody; TNFR80 antibody; TNFRII antibody; Tnfrsf1b antibody; TNR1B_HUMAN antibody; Tumor necrosis factor beta receptor antibody; Tumor necrosis factor binding protein 2 antibody; Tumor necrosis factor receptor 2 antibody; Tumor necrosis factor receptor superfamily member 1B antibody; Tumor necrosis factor receptor type II antibody; Tumor necrosis factor-binding protein 2 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human TNFRSF1B

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

14C4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: HeLa whole cell lysate(30µg), HepG2 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), K562 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), HEK293T whole cell lysate(30µg)
All lanes: TNFRSF1B antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 2min
Immunofluorescence staining of THP-1 cell with CSB-RA159920A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing HT29 cells stained with CSB-RA159920A0HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Description

TNFRSF1B, also known as TNF receptor 2 or CD120b, serves as a critical mediator of tumor necrosis factor signaling with distinct biological functions from its counterpart TNFR1. While TNFR1 is ubiquitously expressed, TNFRSF1B shows more restricted expression patterns and plays specialized roles in immune cell regulation, tissue homeostasis, and inflammatory responses. This receptor has emerged as a significant focus in immunology research, particularly in studies examining autoimmune conditions, cancer immunotherapy, and TNF-mediated signaling pathways.

This recombinant rabbit monoclonal antibody, clone 14C4, offers researchers the consistency and reliability that comes with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures that every lot performs identically, eliminating the variability that can complicate long-term studies or multi-site collaborations. The antibody was raised against a synthetic peptide derived from human TNFRSF1B and has been validated for human samples.

Extensive western blot validation demonstrates robust detection across a diverse panel of human cell lines, including HeLa, HepG2, PC-3, MCF-7, K562, COLO205, and HEK293T lysates. The antibody produces a clean band at the predicted molecular weight of 48 kDa, confirming target specificity. Beyond western blotting, this antibody has been validated for immunofluorescence applications in THP-1 cells and flow cytometry analysis using HT29 cells, providing flexibility across multiple experimental platforms.

Whether investigating TNF signaling mechanisms, characterizing immune cell populations, or exploring TNFRSF1B as a therapeutic target, this antibody delivers the performance and reproducibility essential for meaningful research outcomes.

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Target Background

Function(From Uniprot)

Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

Gene References into Functions

TL1A modulated Rheumatoid arthritis-fibroblast-like synoviocytes migration and Indian hedgehog signaling pathway using TNFR2. PMID:29748156
Data suggest that maternal glycemic response during pregnancy is associated with lower DNA methylation of 4 CpG sites within PDE4B gene in placenta (collected after normal-weight term birth); 3 additional CpG sites are differentially methylated relative to maternal glucose response within TNFRSF1B, LDLR, and BLM genes. (PDE4B = phosphodiesterase-4B; LDLR = low density lipoprotein receptor; BLM = Bloom syndrome protein) PMID:29752424
serum level did not change after tonsillectomy alone but decreased significantly after steroid pulse therapy in patients with IgA nephropathy PMID:28389814
Elevated serum TNFR2 may be a possible marker of COPD in asymptomatic smokers and ex-smokers. PMID:28744116
TNFR2 promoted Adriamycin resistance in breast cancer cells by regulating the DNA damage repair. PMID:28677724
Serum TNFR2 is a biomarker for patients with chronic kidney disease. PMID:28667032
Data indicate activators of tumor necrosis factor receptor 2 (TNFR2) and a potential role for this target in immunotherapy. PMID:27626702
In this study, we identified a novel association between ANCA levels, TNFRSF1B genotype and decreased circulating TNFR2 levels, which may be reflective of the underlying biological mechanisms that determine clinical expression and/or response to certain therapies. PMID:27104820
In Han Chinese population of Hunan province, TNFRSF1B+676 gene polymorphisms are not associated with the genetic risk of rheumatoid arthritis PMID:27640805
Results showed that TNFR2 was upregulated in papillary thyroid carcinoma tissues, and its expression is regulated by H19. PMID:29287713
The TNFRSF1Brs3397 variant may play a role in modulating the risk of rheumatoid arthritis, but does not provide strong evidence of an impact of TNFRSF1B variants in determining response to anti-TNF drugs. PMID:25850964
atopic dermatits patients had increased TNFR2 expression on immune cells PMID:29212072
the blocking of tumor necrosis factor receptor 2 (TNFR2) decreased TL1A-stimulated IL-6 production by rheumatoid arthritis fibroblast-like synoviocytes. PMID:27081759
Data suggest that, by targeting tumor cells and immunosuppressive tumor-associated Tregs, antagonistic tumor necrosis factor receptor 2 (TNFR2) antibodies may be an effective treatment for cancers positive for TNFR2. PMID:28096513
p75 neurotrophin receptor (p75TNFR) was the first NGFR (nerve growth factor receptor) to be cloned and the founding member of the TNFR (tumor necrosis factor receptor) superfamily. However, data suggest that p75TNF is an atypical TNFR superfamily protein; p75TNFR forms dimers (activated by dimeric neurotrophins) that are structurally unrelated to TNFR superfamily proteins. [REVIEW] PMID:28215307
High plasma levels of TNFR2 and TNFR1 were associated with incident intracerebral hemorrhage. PMID:28830973
Serum TNFR2 levels were elevated in lupus nephritis patients versus controls. PMID:27973968
Voxel-based morphometry was used to analyse the associations between TNFRSF1B (rs1061624) genotypes and grey matter structure. Analysis of the TNFRSF1B SNP rs1061624 yielded a significant association with hippocampal but not with striatal volume, whereby G homozygotes were associated with increased volumes relative to A homozygotes and heterozygotes. PMID:27528091
Our results demonstrated that Treg frequencies and TNFR2 expression on Tregs are increased in sarcoidosis, followed by a decline during infliximab therapy, suggesting a pathophysiological role of this T cell subset. PMID:27158798
identified 6 known and 4 novel variations in 6 different exons of TNFR2 gene; out of these identified variations, 5 known variations were found to be significantly associated with the risk of cervical cancer; postmenopausal women having CAAGC + CTGCC haplotypes in TNFR2 gene along with HPV infection and tobacco consumption may lead to the development of cervical cancer PMID:27145290
LTbetaR is essential for efficient liver regeneration and cooperates with TNFRp55 in this process. Differences in survival kinetics strongly suggest distinct functions for these two cytokine receptors in liver regeneration. PMID:26708145
that U87-p75(NTR) cells express higher levels of Cdh-11 protein and that siRNA-mediated knockdown of Cdh-11 resulted in a significant decrease in p75(NTR)-mediated glioblastoma cell migration PMID:26476273
TNF-alpha/TNFR2 regulatory axis stimulates EphB2-mediated neuroregeneration via activation of NF-kappaB. PMID:26492598
This meta-analysis demonstrates that TNFRSF1B T allele carriers show a better response to anti-TNF therapy--{REVIEW} PMID:26071216
Pretransplant recipient circulating CD4+CD127lo/-TNFR2+ Treg cell is potentially a simpler alternative to Treg cell function as a pretransplant recipient immune marker for acute kidney injury. PMID:26425877
NGF has a role in modulating trkANGFR/p75NTR in alphaSMA-expressing conjunctival fibroblasts from human ocular cicatricial pemphigoid PMID:26569118
our findings implicate TNFR2 in supporting myeloid-derived suppressor cells -mediated immune suppression and metastasis in the liver PMID:26483205
In conclusion, we report a significant association between the TNFRSF1B p.M196R variant and the risk for psoriasis and the response to treatment with anti-TNF or anti-Il-12/Il-23. PMID:25537528
Plasma sTNFR2 was higher during pregnancy compared with controls. Urinary levels of sTNFR2 were higher in preeclampsia and pregnant women compared with controls. PMID:25034210
study suggests that TNFR2(+) Tregs play a role in promoting tumor progressive metastasis PMID:26280204
Recurrent point mutations and genomic gains of TNFRSF1B, encoding the tumor necrosis factor receptor TNFR2, are present in a subset of patients with mycosis fungoides and Sezary syndrome. PMID:26258847
Increased plasma level of sTNFRII is found to be associated with exudative age-related macular degeneration. PMID:25363549
ADAM17 was identified as the protease responsible for TNFR2 shedding by CD8(+) T cells, with ADAM17 and TNFR2 required in "cis" for shedding to occur. PMID:26019295
hTNFR2 blocks the biological activity of lymphotoxin beta. PMID:25940088
NRH2 enhanced the ratio of Bax/Bcl-2 by promoting the expressions of proNGF, sortilin and p75NTR, thereby inducing brain cell apoptosis. PMID:25854576
Only TNFR2 can induce TRAF2 degradation. PMID:25152365
Data show that TNF-alpha receptor TNFR2 mRNA expression was significantly increased after 6, 9 and 12 hours of poly (I:C) stimulation. PMID:25419735
Functional TNFR2 196 M/R polymorphism is associated with susceptibility to rheumatoid arthritis in the European population. PMID:24777778
Serum TNFR2 is associated with renal decline and ESRD risk in type 1 diabetes and proteinuria. PMID:24898299
The TNFRII nt587 G/G genotype may increase the risk of developing AS in the Chinese population. PMID:25061744
High TNF receptor 2 is closely associated with the loss of kidney function. PMID:24717758
Inflammation mediated through TNFalpha and its receptors, TNFR1 and TNFR2, may represent an important component of a comorbidity-induced inflammatory response that partially drives the pathophysiology of heart failure (HF) with preserved ejection fraction. PMID:24923671
TNF-TNFR2 signaling may induce RBR in naive BM-EPCs and that blocking TNF-TNFR2 signaling may prevent delayed RBR in BM-EPCs, conceivably, in bone marrow milieu in general PMID:24711449
Blood levels of CRP, IL-6 and TNFalpha-R2 are not associated with incident depression. PMID:24836084
Addition of leukemia inhibitory factor (LIF) neutralizing antibodies inhibited oligodendrocyte differentiation, indicating a crucial role of TNFR2-induced astrocyte derived LIF for oligodendrocyte maturation PMID:24310780
Higher plasma TNFR75 levels were associated with decreased time to first COPD exacerbatino in a prospective study. PMID:24136332
Our results support a role of TNFRSF1B gene variants in the response to IFX in CD patients. PMID:24121042
Serum TNFR2 expression levels might be a powerful prognostic factor for patients treated with the R-CHOP regimen. PMID:23672298
Release of nonmuscle myosin II from the cytosolic domain of tumor necrosis factor receptor 2 is required for target gene expression. PMID:23861542
genetic association studies in a population of women in Tunisia: Data suggest that an SNP in exon 6 of TNFR2/TNFRSF1B (rs1061622) is associated with pre-eclampsia. PMID:23799986