KRT15 Recombinant Monoclonal Antibody

Code: CSB-RA551654A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, IHC

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Product Details
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Target Background

Product Details

Uniprot NO.

P19012

Target Names

KRT15

Alternative Names

Keratin, type I cytoskeletal 15 (Cytokeratin-15) (CK-15) (Keratin-15) (K15), KRT15, KRTB

Species Reactivity

Human

Immunogen

A synthesized peptide from human KRT15 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

11G10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol Immunohistochemistry (IHC) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA551654A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Description

Keratin 15 is a type I intermediate filament protein that serves as a well-established marker for stem cell populations in stratified epithelia, particularly within the hair follicle bulge region. Its expression pattern makes it valuable for researchers investigating epithelial stem cell biology, skin homeostasis, and the cellular origins of various carcinomas. Understanding KRT15 expression dynamics provides important insights into tissue regeneration, wound healing, and cancer stem cell identification.

This recombinant monoclonal antibody, generated from clone 11G10, offers the reproducibility and consistency that demanding research applications require. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, researchers can expect uniform performance across experiments and studies, eliminating the lot-to-lot variability that can complicate long-term projects or multi-site collaborations. The antibody was raised against a synthetic peptide derived from human KRT15, ensuring precise epitope targeting.

Validation in immunohistochemistry demonstrates reliable performance in formalin-fixed, paraffin-embedded human tissue sections. Testing in human lung cancer tissue using a Leica Bond system with citrate buffer antigen retrieval at pH 6.0 confirms compatibility with standard automated staining platforms, with optimal results achieved at 1:100 dilution. This workflow flexibility extends to ELISA applications, with recommended working dilutions between 1:50 and 1:200 for immunohistochemistry depending on specific experimental conditions.

Supplied in a glycerol-containing buffer optimized for long-term stability at -20°C or -80°C, this antibody is well-suited for researchers studying epithelial biology, cancer stem cell populations, hair follicle dynamics, and tumor pathology where accurate KRT15 detection is essential for experimental success.

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Target Background

Gene References into Functions

Underexpression of CK15 is associated with Esophageal Squamous Cell Carcinomas. PMID:27797221
Cytokeratin 15 is upregulated in a subset of urothelial cell carcinomas. PMID:24260555
K15 is expressed variably in both oral squamous cell carcinoma and squamous intraepithelial neoplasm PMID:22648550
both expanded limbal NCs and HUVEC rejoined with LEPC to form spheres to upregulate expression of DeltaNp63alpha, CK15, and CEBPdelta, the former but not the latter abolished expression of CK12 keratin. PMID:22538425
Stromal expression of KRT15, TCN1, and HOXB13 was significantly correlated with tumor grade, stromal hypercellularity, mitotic activity and microscopic borders. PMID:21574054
CK15 highlights BZ cells in normal human follicles PMID:21251039
Physiological concentrations of thyroid hormones T3 and T4 enhance keratin 15 promoter activity and expression in epithelial stem cells of adult human scalp hair follicles in situ and in vitro. PMID:20656376
Report expression of stem-cell markers cytokeratin 15 and nestin in primary adnexal neoplasms. PMID:20700038
Results identify the regulators of keratin 15 promoter activity and their binding sites. PMID:15060141
Cytokeratin 15 expression pattern is useful in terms of distinguishing trichoepithelioma from basal cell carcinoma. PMID:18789069
marker of the hair follicle stem cells; diminished during scarring in alopecia and discoid lupus erythematosus PMID:19162247
Identification of a subset of breast carcinomas characterized by expression of CK15: relationship between CK15+ progenitor/amplified cells and pre-malignant lesions and invasive disease is reported. PMID:19383306
KRT15 is a Gli1-responsive gene in human skin keratinocytes PMID:20050020
KRT15 mRNA is selectively expressed in human hair follicle stem cells, while KRT15 protein is not. PMID:20050020

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Protein Families

Intermediate filament family

Tissue Specificity

Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Exp

Database Links

HGNC: 6421

UNIGENE: Hs.654570

KEGG: hsa:3866

STRING: 9606.ENSP00000254043

OMIM: 148030

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