KRT15 Monoclonal Antibody

CSB-MA785224

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Size:

50μl100μl

US$166

Quantity:

Species Reactivity: Human

Raised in: Mouse

Application: ELISA, IHC

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Target Background

Product Details

Uniprot NO.

P19012

Target Names

KRT15

Alternative Names

AI528832 antibody; CK 15 antibody; CK-15 antibody; CK15 antibody; Cytokeratin-15 antibody; Cytokeratin15 antibody; K15 antibody; K1C15_HUMAN antibody; K1CO antibody; Ka15 antibody; Keratin 15 antibody; Keratin 15 basic antibody; Keratin 15 beta antibody; Keratin antibody; Keratin complex 1 acidic gene 15 antibody; Keratin type I cytoskeletal 15 antibody; Keratin-15 antibody; Keratin15 antibody; KRT 15 antibody; Krt1-15 antibody; KRT15 antibody; KRTB antibody; KRTL15 antibody; Type I cytoskeletal 15 antibody; Type I keratin Ka15 antibody

Raised in

Mouse

Species Reactivity

Human

Immunogen

Synthesized peptide derived from human Cytokeratin 15

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

IgG1, Kappa

Clone No.

17C5

Purification Method

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Protocols

ELISA Protocol Immunohistochemistry (IHC) Protocol

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-MA785224 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-MA785224 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.

Description

Immunization of mice with a synthesized peptide derived from the human KRT15 protein is the first step in the production of the KRT15 monoclonal antibody. B cells isolated from the mouse spleen are fused with myeloma cells to generate hybridomas. Hybridomas are screened to identify those that produce KRT15 antibodies. The chosen hybridomas are cultured in the mouse abdominal cavity, and the KRT15 monoclonal antibodies are purified using affinity chromatography with a specific immunogen. The resulting KRT15 monoclonal antibody is a mouse monoclonal antibody that binds specifically to human KRT15 protein and can be used for ELISA and IHC applications.

KRT15 is a type I intermediate filament protein that is mainly expressed in basal cells of the skin and other stratified epithelia. Its main function is to provide structural support to cells and tissues, and it plays a critical role in maintaining the integrity and strength of the skin. In addition, KRT15 has been implicated in the regulation of cell proliferation, differentiation, and migration, as well as wound healing and tissue repair processes. Mutations in the KRT15 gene have been linked to various skin disorders, including epidermolysis bullosa simplex and other keratinopathies.

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Target Background

Gene References into Functions

Underexpression of CK15 is associated with Esophageal Squamous Cell Carcinomas. PMID:27797221
Cytokeratin 15 is upregulated in a subset of urothelial cell carcinomas. PMID:24260555
K15 is expressed variably in both oral squamous cell carcinoma and squamous intraepithelial neoplasm PMID:22648550
both expanded limbal NCs and HUVEC rejoined with LEPC to form spheres to upregulate expression of DeltaNp63alpha, CK15, and CEBPdelta, the former but not the latter abolished expression of CK12 keratin. PMID:22538425
Stromal expression of KRT15, TCN1, and HOXB13 was significantly correlated with tumor grade, stromal hypercellularity, mitotic activity and microscopic borders. PMID:21574054
CK15 highlights BZ cells in normal human follicles PMID:21251039
Physiological concentrations of thyroid hormones T3 and T4 enhance keratin 15 promoter activity and expression in epithelial stem cells of adult human scalp hair follicles in situ and in vitro. PMID:20656376
Report expression of stem-cell markers cytokeratin 15 and nestin in primary adnexal neoplasms. PMID:20700038
Results identify the regulators of keratin 15 promoter activity and their binding sites. PMID:15060141
Cytokeratin 15 expression pattern is useful in terms of distinguishing trichoepithelioma from basal cell carcinoma. PMID:18789069
marker of the hair follicle stem cells; diminished during scarring in alopecia and discoid lupus erythematosus PMID:19162247
Identification of a subset of breast carcinomas characterized by expression of CK15: relationship between CK15+ progenitor/amplified cells and pre-malignant lesions and invasive disease is reported. PMID:19383306
KRT15 is a Gli1-responsive gene in human skin keratinocytes PMID:20050020
KRT15 mRNA is selectively expressed in human hair follicle stem cells, while KRT15 protein is not. PMID:20050020

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Protein Families

Intermediate filament family

Tissue Specificity

Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Exp

Database Links

HGNC: 6421

UNIGENE: Hs.654570

KEGG: hsa:3866

STRING: 9606.ENSP00000254043

OMIM: 148030

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