Abbreviation
Recombinant Human TNF protein, partial (Active)
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human TNF at 5 μg/mL can bind Human TNFR2 protein (CSB-MP023978HU2). The EC50 is 3.470-4.107 ng/mL.
Alternative Names
APC1; APC1 protein; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; Tnf; TNF superfamily member 2; TNF superfamily, member 2; TNF, macrophage derived; TNF, monocyte derived; TNF-a; TNF-alpha; TNFA; TNFA_HUMAN; TNFSF2; Tumor necrosis factor (TNF superfamily member 2); Tumor necrosis factor alpha; Tumor necrosis factor; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor, Membrane Form; Tumor necrosis factor, soluble form
Species
Homo sapiens (Human)
Expression Region
77-233aa
Target Protein Sequence
VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
N-terminal 6xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Tris-based buffer,50% glycerol
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human TNF at 5 μg/ml can bind Human TNFR2 protein (CSB-MP023978HU2). The EC50 is 3.470-4.107 ng/mL.
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Description
TNF-α drives NF-κB and MAPK signaling cascades central to inflammation, apoptosis, and immune cell activation, making validated binding activity essential for meaningful downstream results. This recombinant human TNF (aa 77–233) demonstrates potent receptor engagement, with an EC50 of 3.470–4.107 ng/mL for human TNFR2 binding in functional ELISA, supporting its use as a coating antigen in antibody development, a positive control in cytokine detection assays, and a reference ligand in receptor-binding studies. Expressed in yeast with an N-terminal 6xHis tag, the protein covers the mature soluble domain and achieves greater than 90% purity by SDS-PAGE, which aligns with standards expected in cell-based activation and apoptosis assays; however, researchers should note that endotoxin levels have not been tested, so independent screening is advisable before use in sensitive immune cell experiments such as PBMC stimulation or primary T-cell proliferation studies.
