Abbreviation
Recombinant Human CD96 protein, partial (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized CD96 at 2 μg/mL can bind anti-CD96 antibody （CSB-RA004971A0HU）, the EC50 is 95.78-127.1 ng/mL.
Alternative Names
(Cell surface antigen CD96)(T cell-activated increased late expression protein)(CD antigen CD96)
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
22-503aa
Target Protein Sequence
VWEKTVNTEENVYATLGSDVNLTCQTQTVGFFVQMQWSKVTNKIDLIAVYHPQYGFYCAYGRPCESLVTFTETPENGSKWTLHLRNMSCSVSGRYECMLVLYPEGIQTKIYNLLIQTHVTADEWNSNHTIEIEINQTLEIPCFQNSSSKISSEFTYAWSVEDNGTQETLISQNHLISNSTLLKDRVKLGTDYRLHLSPVQIFDDGRKFSCHIRVGPNKILRSSTTVKVFAKPEIPVIVENNSTDVLVERRFTCLLKNVFPKANITWFIDGSFLHDEKEGIYITNEERKGKDGFLELKSVLTRVHSNKPAQSDNLTIWCMALSPVPGNKVWNISSEKITFLLGSEISSTDPPLSVTESTLDTQPSPASSVSPARYPATSSVTLVDVSALRPNTTPQPSNSSMTTRGFNYPWTSSGTDTKKSVSRIPSETYSSSPSGAGSTLHDNVFTSTARAFSEVPTTANGSTKTNHVHITGIVVNKPKDGM
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Partial of Isoform 2
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
-
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
-
Activity
Measured by its binding ability in a functional ELISA. Immobilized CD96 at 2 μg/ml can bind anti-CD96 antibody （CSB-RA004971A0HU）, the EC50 is 95.78-127.1 ng/mL.
Description
CD96 functions as an emerging immune checkpoint receptor that competes with TIGIT for binding to shared ligands, making it a compelling target in immuno-oncology and T-cell regulation studies. This recombinant human CD96 construct covers the extracellular region (aa 22–503 of Isoform 2), encompassing the ligand-binding immunoglobulin-like domains, and is expressed in mammalian cells to preserve native glycosylation and conformational integrity critical for receptor-ligand interaction assays. Functional ELISA validation confirms robust binding activity, with immobilized CD96 at 2 μg/mL engaging anti-CD96 antibody at an EC50 of 95.78–127.1 ng/mL, supporting use as a positive control in binding assays, competitive blocking antibody screening, and therapeutic antibody epitope mapping. The C-terminal 10×His tag facilitates oriented immobilization for SPR or BLI affinity characterization, while purity exceeding 95% and endotoxin levels below 1.0 EU/μg satisfy the criteria typically required for sensitive immune checkpoint research and small-molecule inhibitor screening campaigns.
-SDS.jpg)
-AC1.jpg)
.svg)
