Abbreviation
Recombinant Human PTH protein, partial (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to induce cAMP accumulation in murine MC3T3E1 cells is less than 50 ng/ml, corresponding to a specific activity of >2.0x104 IU/mg.
Research Area
Signal Transduction
Alternative Names
hPTH; Parathormone; Parathyrin; Parathyroid hormone 1; Parathyroid hormone; Prepro PTH; Preproparathyroid hormone; PTH; PTH1; PTH1 receptor; PTH1R; PTHR; PTHR1; PTHY_HUMAN
Species
Homo sapiens (Human)
Expression Region
32-65aa
Target Protein Sequence
SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNF
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
This tag-free fragment spanning residues 32–65 of human PTH retains the biologically active N-terminal signaling domain critical for PTH1 receptor engagement and downstream cAMP generation. Validated in a cell-based assay, the protein induces cAMP accumulation in murine MC3T3E1 osteoblastic cells with an ED50 below 50 ng/ml, corresponding to a specific activity exceeding 2.0 × 10⁴ IU/mg—providing a reliable positive control for receptor binding assays, competition studies, and cell-based signaling experiments. Endotoxin levels below 1.0 EU/μg, combined with purity greater than 97% by SDS-PAGE, satisfy the criteria typically required for in vivo pharmacology models investigating calcium homeostasis or bone metabolism, as well as for use as a quantitative standard in immunoassay development. The E. coli-derived, tag-free format supports structure-function studies where post-translational modifications or fusion partners could confound receptor interaction analyses.
