Abbreviation
Recombinant Human FGF7 protein (Active)
Purity
>96% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10 ng/ml, corresponding to a specific activity of >1.0x105 IU/mg.
Alternative Names
FGF 7; FGF-7; Fgf7; FGF7_HUMAN; Fibroblast growth factor 7; HBGF 7; HBGF-7; HBGF7; Heparin binding growth factor 7; Heparin-binding growth factor 7; Keratinocyte growth factor; KGF
Species
Homo sapiens (Human)
Expression Region
32-194aa
Target Protein Sequence
CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
0.2 μm filtered 20 mM PB, 0.5 M NaCl, pH 8.0 ,lyophilized
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
FGF7, also known as keratinocyte growth factor, plays a critical role in epithelial cell proliferation and tissue repair, making it a key tool in wound healing models and tumor biology research. This tag-free recombinant form covers the full mature sequence (residues 32–194) and demonstrates strong receptor-binding potency, with an ED50 below 10 ng/ml in a thymidine uptake assay using FGF receptor-transfected BaF3 cells, corresponding to a specific activity exceeding 1.0 × 10⁵ IU/mg — data that supports use in cell proliferation assays, receptor-ligand binding studies via SPR or ELISA, and in vivo regenerative medicine models. Produced in *E. coli*, the protein lacks glycosylation, which provides a homogeneous preparation well suited for quantitative applications such as ELISA standards and antibody characterization where post-translational variability could confound results. Purity exceeding 96% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for cell-based functional assays and in vivo studies.
