CDH1, a calcium-dependent cell adhesion molecule, is predominantly expressed in epithelial cells. CDH1 plays a crucial role in maintaining the structural integrity of these tissues. Its transmembrane and extracellular cadherin repeat domains enable homotypic cell adhesion, which is vital for tissue development, cell migration, proliferation control, and the maintenance of epithelial barrier function. Dysregulation of CDH1 expression is implicated in various diseases, especially cancer, where it influences tumor initiation, progression, invasion, and metastasis. As a tumor suppressor, CDH1's deficiency or dysfunction can lead to reduced cellular adhesion and increased cell mobility, promoting cells invasion and spread in cancer. The roles of CDH1 in cancer makes it a potential therapeutic target and a prognostic marker for cancers.
Popular
Product:
Recombinant Human Cadherin-1(CDH1),partial (Active)(CSB-MP005034HU1)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CDH1 at 2μg/mL can bind Anti-CDH1 recombinant antibody(CSB-RA005034MA2HU)，the EC50 is 5.151-6.358 ng/mL.
CDH1 Recombinant Monoclonal Antibody(CSB-RA005034MA1HU)
Validated Data
Western Blot
Positive WB detected in: Mouse Liver tissue lysate
All lanes: CDH1 antibody at 1:500
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 80-135 kDa
Observed band size: 135 kDa
IHC image of CSB-RA005034MA1HU diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing MCF7 cells stained with CSB-RA005034MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,026 events was performed.
The following CDH1
reagents supplied by CUSABIO are manufactured under a
strict quality control system. Multiple applications
have been validated and solid technical support is
offered.
CDH1 Proteins
CDH1 Proteins for Gallus gallus (Chicken)
CDH1 Proteins for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
CDH1 Proteins for Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
CDH1 Proteins for Rattus norvegicus (Rat)
CDH1 Proteins for Homo sapiens (Human)
CDH1 Proteins for Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
CDH1 Proteins for Bos taurus (Bovine)
CDH1 Proteins for Mus musculus (Mouse)
CDH1 Proteins for Xenopus laevis (African clawed frog)
CDH1 Proteins for Dictyostelium discoideum (Slime mold)
