Abbreviation
Recombinant Human TNFSF15 protein, partial (Active)
Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to induce apoptosis using human TF-1 cells is less than 20 ng/ml, corresponding to a specific activity of >5.0x104 IU/mg.
Alternative Names
TNFSF15; TL1; VEGI; Tumor necrosis factor ligand superfamily member 15; TNF ligand-related molecule 1; Vascular endothelial cell growth inhibitor
Species
Homo sapiens (Human)
Expression Region
72-251aa
Target Protein Sequence
LKGQEFAPSH QQVYAPLRAD GDKPRAHLTV VRQTPTQHFK NQFPALHWEH ELGLAFTKNR MNYTNKFLLI PESGDYFIYS QVTFRGMTSE CSEIRQAGRP NKPDSITVVI TKVTDSYPEP TQLLMGTKSV CEVGSNWFQP IYLGAMFSLQ EGDKLMVNVS DISLVDYTKE DKTFFGAFLL
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Tag Info
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
0.2 μm filtered PBS, pH 7.4, with 0.02% Tween-20 ,lyophilized
Lead Time
5-10 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Datasheet & COA
Please contact us to get it.
Description
TNFSF15 (also known as TL1A) plays a critical role in modulating immune responses and promoting apoptosis in tumor biology, making it a valuable tool for cancer-related functional studies. This tag-free recombinant human TNFSF15, spanning residues 72–251, demonstrates potent pro-apoptotic activity with an ED50 of less than 20 ng/mL in TF-1 cell apoptosis assays, corresponding to a specific activity exceeding 5.0 × 10⁴ IU/mg—providing a reliable reference for cell-based functional assays, NF-κB and MAPK signaling pathway dissection, and in vivo tumor biology models. Endotoxin levels below 1.0 EU/μg ensure that apoptotic and inflammatory readouts reflect genuine TNFSF15 signaling rather than LPS-driven artifacts, which is essential when working with sensitive immune cell populations. Purity exceeding 97% by SDS-PAGE, combined with this stringent endotoxin control, supports use as a positive control in cytokine detection assays and as an immunogen or coating antigen for antibody development campaigns.
