MRGPRX2 Recombinant Monoclonal Antibody

Code: CSB-RA839361MA1HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, IHC

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Target Background

Product Details

Uniprot NO.

Q96LB1

Target Names

MRGPRX2

Alternative Names

G protein coupled receptor MRGX 2 antibody; G protein coupled receptor MRGX2 antibody; Mas related G protein coupled receptor member X2 antibody; Mas related gene MRGX 2 antibody; Mas related gene MRGX2 antibody; MAS related GPR member X2 antibody; Mas-related G-protein coupled receptor member X antibody; Mas-related G-protein coupled receptor member X2 antibody; MRGPRX 2 antibody; MRGPRX2 antibody; MRGX 2 antibody; MRGX2 antibody; MRGX2_HUMAN antibody

Species Reactivity

Human

Immunogen

Recombinant Human MRGPRX2 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

6C3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA839361MA1HU diluted at 1:50 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.

Description

MRGPRX2 (Mas-related G-protein coupled receptor member X2) has emerged as a critical player in understanding non-IgE-mediated mast cell activation, making it a compelling target for researchers investigating pseudo-allergic drug reactions, chronic urticaria, and neurogenic inflammation. This receptor's ability to trigger mast cell degranulation in response to diverse ligands—including certain FDA-approved drugs, neuropeptides, and antimicrobial peptides—positions it at the intersection of immunology, pharmacology, and neuroscience research.

This recombinant monoclonal antibody (clone 6C3) offers the reproducibility that demanding experimental workflows require. Because recombinant antibodies are produced from defined genetic sequences rather than hybridoma supernatants, researchers benefit from consistent lot-to-lot performance, eliminating a common source of experimental variability when studying this relatively recently characterized receptor.

Validation in human skin tissue demonstrates this antibody's utility for investigating MRGPRX2 in its native physiological context. The immunohistochemistry protocol, optimized at 1:50-1:200 dilution using citrate buffer antigen retrieval on paraffin-embedded sections, provides a reliable starting point for researchers examining receptor distribution in cutaneous mast cells—a population of particular interest given skin's role as a primary site of pseudo-allergic reactions. The antibody has also been validated for ELISA applications, offering flexibility for quantitative studies.

Supplied in a glycerol-based PBS buffer with Proclin 300 preservative, this human IgG1-format antibody arrives ready for immediate use in your experimental pipeline. For investigators exploring mast cell biology, drug-induced hypersensitivity mechanisms, or the broader landscape of GPCR signaling in sensory neurons, this antibody provides a sequence-defined tool for advancing MRGPRX2 research.

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Target Background

Function(From Uniprot)

Mast cell-specific receptor for basic secretagogues, i.e. cationic amphiphilic drugs, as well as endo- or exogenous peptides, consisting of a basic head group and a hydrophobic core. Recognizes and binds small molecules containing a cyclized tetrahydroisoquinoline (THIQ), such as non-steroidal neuromuscular blocking drugs (NMBDs), including tubocurarine and atracurium. In response to these compounds, mediates pseudo-allergic reactions characterized by histamine release, inflammation and airway contraction. Acts as a receptor for a number of other ligands, including peptides and alkaloids, such as cortistatin-14, proadrenomedullin N-terminal peptides PAMP-12 and, at lower extent, PAMP-20, antibacterial protein LL-37, PMX-53 peptide, beta-defensins, and complanadine A.

Gene References into Functions

Low levels of MRGPRX2 were detected in non-asthma lung mast cells, but its expression was significantly up-regulated in asthma lung mast cells. PMID:29295703
the present study demonstrates that FceRI and MRGPRX2 function can be inversely regulated by SCF in an acute scenario, suggesting that the signaling requirements differ profoundly between the routes. PMID:28859248
The authors discuss the possibility that MRGPRX2 responds to various as-yet-unidentified endogenous ligands that have specific characteristics, and propose that MRGPRX2 plays an important role in regulating inflammatory responses to endogenous harmful stimuli, such as protein breakdown products released from damaged or dying cells. PMID:29484687
these peptides are identified as fragments of albumin. The isolated fragments activate MRGPRX2 and degranulate MRGPRX2 expressing LAD 2 cells in dose-dependent manner. The isolated basic peptides generated from human albumin are able to degranulate mast cells via the MRGPRX2. PMID:28844982
The results showed a kind of mechanism that sinomenine-induced anaphylactoid reactions were mediated by MRGPRX2 via activating PLC molecular signaling pathways to provoke mast cells Ca(2+) mobilization and degranulation. PMID:28987593
data suggest that mast cells play important roles in both gingival homeostasis and periodontal disease by orchestrating an interaction between gingival epithelial cells and neutrophils via MRGPRX2-mediated degranulation PMID:28694291
MRGX2 is related to nociception, adrenal gland secretion and mast cell degranulation. (Review) PMID:26106044
contributes to larger and longer lasting wheal reactions to intradermally injected neuropeptides in patients with chronic urticaria PMID:24954276
Ectopic expression of MrgX2 in rat basophilic leukemia RBL-2H3 cells and murine bone marrow mast cells renders these cells responsive to human beta-defensin for degranulation. PMID:23698749
identified MrgX2 as a novel G protein-coupled receptor for the antibacterial peptide LL-37 and demonstrated that unlike most G protein-coupled receptors it is resistant to agonist-induced receptor phosphorylation, desensitization, and internalization. PMID:22069323
cortistatin-14 shown to be a high affinity ligand for G protein coupled receptor MrgX2 which is expressed in dorsal root ganglion PMID:12915402
MRGX2 amino acid substitutions may alter the interactions between MRGX2 protein and its ligand, thus, potentially led to adaptive changes of the pain-perception-related nervous system during human evolution. PMID:15862286

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 1 family, Mas subfamily

Tissue Specificity

Mainly expressed in mast cells. Has a limited expression profile, both peripheral and within the central nervous system, with highest levels in dorsal root ganglion. Detected in blood vessels, scattered lymphocytes, and gastrointestinal ganglia (at protei

Database Links

HGNC: 17983

UNIGENE: Hs.350566

KEGG: hsa:117194

STRING: 9606.ENSP00000333800

OMIM: 607228

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