MELTF Recombinant Monoclonal Antibody

Code: CSB-RA013754MA1HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA

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Target Background

Product Details

Uniprot NO.

P08582

Target Names

MELTF

Alternative Names

Melanotransferrin (Melanoma-associated antigen p97) (CD antigen CD228), MELTF, MAP97 MFI2

Species Reactivity

Human

Immunogen

Recombinant Human MELTF protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

4A11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol

Recommended Dilution

Application	Recommended Dilution
ELISA	1:5000-1:50000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

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The Binding Activity of Human MELTF with Anti-MELTF Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human MELTF (CSB-MP013754HU1) at 2 μg/mL can bind Anti-MELTF recombinant antibody. The EC₅₀ is 0.3476-0.4529 ng/mL.

Description

Melanotransferrin, encoded by the MELTF gene and also designated as CD228, represents a fascinating member of the transferrin family with distinctive membrane-anchored properties. Originally identified as melanoma-associated antigen p97, this glycoprotein has garnered significant research interest due to its elevated expression in melanoma cells and its potential roles in iron metabolism, cell proliferation, and tumor progression. Understanding MELTF biology has become increasingly relevant for researchers investigating cancer biomarkers, iron homeostasis, and therapeutic targeting strategies.

This recombinant monoclonal antibody against human MELTF offers the reproducibility and consistency that demanding research applications require. Developed using recombinant technology with clone 4A11, this antibody provides a sequence-defined reagent that eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies. The human IgG1 isotype format and affinity chromatography purification ensure high purity suitable for sensitive detection methods.

Validation studies demonstrate excellent binding characteristics in ELISA applications, with the antibody recognizing immobilized human MELTF protein at concentrations as low as the sub-nanogram range. Functional ELISA testing revealed an EC50 between 0.3476 and 0.4529 ng/mL, indicating strong binding affinity that supports reliable detection even at high dilutions ranging from 1:5000 to 1:50000.

Supplied in a liquid format containing PBS with glycerol for stability, this antibody is ready for immediate use in your experimental workflows. For researchers investigating melanoma biology, transferrin family proteins, or developing diagnostic approaches targeting CD228, this recombinant antibody provides a dependable tool for quantitative ELISA-based detection of human MELTF.

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Target Background

Function(From Uniprot)

Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.

Gene References into Functions

Melanotransferrin is a serological marker of colorectal cancer by secretome analysis and quantitative proteomics. PMID:25216327
PC3 tumors are sustained by a small number of tumor-initiating cells with stem-like characteristics, including strong self-renewal and pro-angiogenic capability and marked by the expression pattern FAM65Bhigh/MFI2low/LEF1low. PMID:21190562
The human genome contains a second melanotransferrin gene (MTf2). There are two isoforms of melanotransferrin. PMID:11852110
its ability to donate 59Fe to SK-Mel-28 melanoma cells and other cell types PMID:12230555
membrane-bound and soluble p97 affect the migration capacity of endothelial and melanoma cells and suggest that p97 could be involved in the regulation of plasminogen activation by interacting with pro-uPA and plasminogen. PMID:12750156
Serum melanotransferrin exists in various conformations depending on the binding of bivalent cations or following post-translational modification; serum melanotransferrin levels are unchanged in subjects with Alzheimer's disease. PMID:12809550
The distribution of MTf and its splice variants may provide clues to their possible biological roles in melanoma and other tumors. PMID:15716025
melanotransferrin has a role in cell proliferation and tumorigenesis PMID:16704991
Anti-angiogenic properties of soluble melanotransferrin may result from local overstimulation of plasminogen activation by tissue plasminogen activator, thus leading to subsequent degradation of the fibronectin matrix and endothelial cell detachment. PMID:16713448
Altogether, these findings strongly suggest that MTf overexpression in melanoma cells contributes to tumor progression by stimulating plasmin generation as well as cell migration and invasion. PMID:17196552
HMB-45 (melanoma-associated antigen p97) and Melan-A combined were positive in 100% of the renal angiomyolipomas PMID:17227112
Novel molecular targets directly or indirectly regulated by MTf and the potential pathways involved in its function, including modulation of proliferation. PMID:17449903
this review discusses the studies that have demonstrated that melanotransferrin is not involved in iron metabolism, but plays a vital role in melanoma cell proliferation and tumorigenesis. PMID:17452986
Biochemical and spectroscopic studies of MTF1 are reported. PMID:18691669

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Subcellular Location

[Isoform 1]: Cell membrane; Lipid-anchor, GPI-anchor.

Protein Families

Transferrin family

Tissue Specificity

Found predominantly in human melanomas and in certain fetal tissues; also found in liver, epithelium, umbilical chord, placenta and sweat gland ducts.

Database Links

HGNC: 7037

UNIGENE: Hs.184727

KEGG: hsa:4241

STRING: 9606.ENSP00000296350

OMIM: 155750

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