Abbreviation
Recombinant Human MELTF protein (Active)
Purity
Greater than 95% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human MELTF at 2 μg/mL can bind Anti-MELTF recombinant antibody (CSB-RA013754MA1HU). The EC50 is 0.3476-0.4529 ng/mL.
Alternative Names
Melanotransferrin; Melanoma-associated antigen p97; CD228; MELTF; MAP97, MFI2
Species
Homo sapiens (Human)
Expression Region
20-709aa
Target Protein Sequence
GMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSADHCVQLIAAQEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGWNVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDGKTLPSWGQALLSQDFELLCRDGSRADVTEWRQCHLARVPAHAVVVRADTDGGLIFRLLNEGQRLFSHEGSSFQMFSSEAYGQKDLLFKDSTSELVPIATQTYEAWLGHEYLHAMKGLLCDPNRLPPYLRWCVLSTPEIQKCGDMAVAFRRQRLKPEIQCVSAKSPQHCMERIQAEQVDAVTLSGEDIYTAGKTYGLVPAAGEHYAPEDSSNSYYVVAVVRRDSSHAFTLDELRGKRSCHAGFGSPAGWDVPVGALIQRGFIRPKDCDVLTAVSEFFNASCVPVNNPKNYPSSLCALCVGDEQGRNKCVGNSQERYYGYRGAFRCLVENAGDVAFVRHTTVFDNTNGHNSEPWAAELRSEDYELLCPNGARAEVSQFAACNLAQIPPHAVMVRPDTNIFTVYGLLDKAQDLFGDDHNKNGFKMFDSSNYHGQDLLFKDATVRAVPVGEKTTYRGWLGLDYVAALEGMSSQQC
Note: The complete
sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is
translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application,
please explicitly request the full and complete sequence of this protein before ordering.
Protein Length
Full Length of Mature Protein of Isoform 1
Tag Info
C-terminal 10xHis-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Storage
Store at -20°C/-81°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Lead Time
3-7 business days
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Shelf Life
The shelf life is related to many factors, storage state, storage temperature and the stability of the product itself. Generally, the shelf life of lyophilized form is 6 months at -20°C/-80°C from the date of receipt.
Datasheet & COA
Please contact us to get it.
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Activity
Measured by its binding ability in a functional ELISA. Immobilized Human MELTF at 2 μg/ml can bind Anti-MELTF recombinant antibody (CSB-RA013754MA1HU). The EC50 is 0.3476-0.4529 ng/mL.
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The purity of MELTF was greater than 95% as determined by SEC-HPLC
Description
Melanotransferrin functions as a membrane-bound iron-binding glycoprotein implicated in iron homeostasis and neural development, making it a valuable target for investigating metal transport mechanisms and melanoma biology. This full-length mature protein (aa 20–709) demonstrates robust antibody-binding activity with an EC50 of 0.3476–0.4529 ng/mL in functional ELISA, confirming that the recombinant construct retains the conformational epitopes required for antibody development, immunoassay standardization, and ligand-binding studies. The protein meets the quality thresholds commonly required for drug-protein interaction assays and membrane transporter functional studies, with purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg. Researchers examining iron sequestration pathways, characterizing MELTF-specific monoclonal antibodies, or performing structural analyses of transferrin family members will find this mammalian-expressed protein provides a suitable basis for experiments demanding native glycosylation and proper disulfide bond formation.
