GPA33 Recombinant Monoclonal Antibody

Code: CSB-RA245537A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, IHC

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Product Details
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Target Background

Product Details

Uniprot NO.

Q99795

Target Names

GPA33

Alternative Names

Cell surface A33 antigen (Glycoprotein A33), GPA33

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human GPA33

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4H4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA245537A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA245537A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Description

GPA33, also known as glycoprotein A33, is a transmembrane protein predominantly expressed in normal intestinal epithelium and maintained in over 95% of colorectal carcinomas. This restricted expression pattern, combined with its stable presence on the cell surface of malignant cells, has established GPA33 as a compelling target for colorectal cancer research and therapeutic antibody development. Understanding GPA33 expression and localization provides valuable insights into intestinal biology and colorectal cancer progression.

This recombinant monoclonal antibody, clone 4H4, offers the reproducibility and consistency that demanding research applications require. Generated against a synthetic peptide derived from human GPA33 and produced using recombinant technology, this antibody delivers sequence-defined specificity with minimal lot-to-lot variation. For researchers conducting longitudinal studies or multi-site collaborations, this consistency translates directly into comparable results across experiments and reduced time spent on antibody re-optimization.

Validation studies demonstrate reliable performance in immunohistochemistry applications on human tissues. Testing on paraffin-embedded human small intestine tissue confirms detection of GPA33 in its expected physiological context, while staining of human colon cancer tissue validates utility in oncology research settings. Both applications performed optimally at 1:100 dilution using citrate buffer antigen retrieval, providing a solid starting point for your experimental optimization.

The antibody is supplied in a glycerol-containing buffer formulation designed for long-term stability at -20°C or -80°C, supporting extended research programs. Whether you are investigating intestinal epithelial biology, characterizing colorectal tumor samples, or evaluating GPA33 as a therapeutic target, this recombinant antibody provides the specificity and consistency essential for meaningful immunological and cancer research outcomes.

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Target Background

Function(From Uniprot)

May play a role in cell-cell recognition and signaling.

Gene References into Functions

A33 shows similar sensitivity to but is more specific than CDX2 as an immunomarker of colorectal carcinoma PMID:28226180
A33 expression is directly correlated to colorectal tumor differentiation. PMID:27272411
Data indicate that EpCAM- and A33-Exos were released from the apical and basolateral surfaces, respectively. PMID:23230278
Data indicate positive staining was observed for both A33 expression and KRN330 binding from patients biopsied on Day 8, 9 or 14 following initial dosing of 3 mg/kg. PMID:23294608
The A33-dependent incorporation of B5 into extracellular enveloped vaccinia virions is mediated through an interaction between their lumenal domains. PMID:22623777
Increased interaction between vaccinia virus proteins A33 and B5 is detrimental to infectious extracellular enveloped virion production. PMID:22623782
cloning, chromosomal localizations, exon-intron structures and transcription start sites - target gene for the intestine-specific homeobox transcription factor, CDX1. PMID:12114523
Regulation of A33 antigen expression by GKLF. PMID:12853980
This work has demonstrated that the antigen is both highly immobile and extremely persistent-retaining its surface localization for a turnover halflife of greater than 2 days. PMID:18236042