GPA33 Antibody

Code: CSB-PA857459EA01HU

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US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IHC, IF

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Product Details
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Target Background

Product Details

Uniprot NO.

Q99795

Target Names

GPA33

Alternative Names

A33 antibody; Cell surface A33 antigen antibody; Cell surface A33 antigen precursor antibody; Glycoprotein A33 (transmembrane) antibody; Glycoprotein A33 antibody; Gpa33 antibody; GPA33_HUMAN antibody; MGC129986 antibody; MGC129987 antibody; OTTHUMP00000032346 antibody; Transmembrane glycoprotein A33 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Cell surface A33 antigen protein (22-235AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Immunofluorescence staining of MCF-7 cells with CSB-PA857459EA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western Blot
Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate
All lanes: GPA33 antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 50 kDa
Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA857459EA01HU at dilution of 1:100

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Target Background

Function(From Uniprot)

May play a role in cell-cell recognition and signaling.

Gene References into Functions

A33 shows similar sensitivity to but is more specific than CDX2 as an immunomarker of colorectal carcinoma PMID:28226180
A33 expression is directly correlated to colorectal tumor differentiation. PMID:27272411
Data indicate that EpCAM- and A33-Exos were released from the apical and basolateral surfaces, respectively. PMID:23230278
Data indicate positive staining was observed for both A33 expression and KRN330 binding from patients biopsied on Day 8, 9 or 14 following initial dosing of 3 mg/kg. PMID:23294608
The A33-dependent incorporation of B5 into extracellular enveloped vaccinia virions is mediated through an interaction between their lumenal domains. PMID:22623777
Increased interaction between vaccinia virus proteins A33 and B5 is detrimental to infectious extracellular enveloped virion production. PMID:22623782
cloning, chromosomal localizations, exon-intron structures and transcription start sites - target gene for the intestine-specific homeobox transcription factor, CDX1. PMID:12114523
Regulation of A33 antigen expression by GKLF. PMID:12853980
This work has demonstrated that the antigen is both highly immobile and extremely persistent-retaining its surface localization for a turnover halflife of greater than 2 days. PMID:18236042