GIPR Recombinant Monoclonal Antibody

Code: CSB-RA009438MA2HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, WB

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Target Background

Product Details

Uniprot NO.

P48546

Target Names

GIPR

Alternative Names

Gastric inhibitory polypeptide receptor, GIPR

Species Reactivity

Human

Immunogen

Recombinant Human GIPR protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Human IgG

Clone No.

32C2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Western Blotting(WB) Protocol

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in:Hela whole cell lysate, PC-3 whole cell lysate, U251 whole cell lysate, HepG2 whole cell lysate
All lanes: GIPR antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/50000 dilution
Predicted band size: 54 kDa,49 kDa,50 kDa
Observed band size: 54 kDa

Description

Gastric inhibitory polypeptide receptor (GIPR) plays a central role in metabolic signaling, mediating the incretin effect that enhances glucose-dependent insulin secretion following nutrient intake. As a G protein-coupled receptor expressed in pancreatic beta cells, adipose tissue, and the central nervous system, GIPR has emerged as a compelling therapeutic target in diabetes and obesity research, particularly with the recent success of dual GIP/GLP-1 receptor agonists in clinical applications.

This recombinant monoclonal antibody (clone 32C2) offers the reproducibility essential for longitudinal studies and multi-site collaborations. Because it is generated from a defined sequence rather than traditional hybridoma methods, researchers benefit from consistent performance across lots, eliminating the variability that can compromise experimental comparisons over time. The human IgG1 isotype format and affinity-chromatography purification ensure high specificity for your target with minimal background interference.

Validation studies confirm reliable detection of human GIPR across diverse cellular contexts. Western blot analysis demonstrates clear detection at the predicted 54 kDa molecular weight in HeLa, PC-3, U251, and HepG2 whole cell lysates, with optimal performance at dilutions ranging from 1:500 to 1:5000. This broad compatibility across epithelial, prostate carcinoma, glioblastoma, and hepatocellular carcinoma cell lines provides flexibility for researchers investigating GIPR expression in various disease models. The antibody is also validated for ELISA applications, supporting both quantitative expression studies and screening workflows.

Whether you are investigating incretin signaling pathways, characterizing GIPR expression in metabolic disease models, or developing screening assays for receptor-targeted therapeutics, this antibody delivers the consistency and validated performance your research demands.

Customer Reviews and Q&A

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Target Background

Function(From Uniprot)

This is a receptor for GIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

Gene References into Functions

an association between the GIPR rs2302382 polymorphism and type 2 diabetes mellitus in Egyptian patients. PMID:28744963
Data suggest that GIPR (gastric inhibitory polypeptide receptor) is among the few GPCRs (G-protein-coupled receptors) which signal through G-proteins (GTP-binding protein Gs alpha subunit, here) both at plasma membrane and in endosomes; recombinant proteins expressed in HEK293 cells were used in these studies. PMID:27641811
GIPR overexpression does not appear to affect acromegalic patients' clinical features PMID:28179449
Study shows the internalization of GIPR involving clathrin-coated pits, AP-2 and dynamin and its subsequent intracellular trafficking. GIP stimulates a rapid robust internalization of the GIPR, the major part being directed to lysosomes. PMID:26225752
The common variant rs10423928 in the GIPR gene is associated with increased risk of stroke in patients with type 2 diabetes. PMID:26395740
Body mass index change for the A/T+A/A in GIPR genotypes was significantly higher than that for the T/T genotype. rs10423928 may predict weight gain in schizophrenia. PMID:25321336
The potential future role of gastric inhibitory peptide (GIP) receptors as molecular targets in neuroendocrine neoplasms may be dependent on the tumor grade. PMID:25591947
Results show that GIPR undergoes trafficking between the plasma membrane and intracellular compartments of both GIP-stimulated and unstimulated adipocytes. PMID:25047836
GIPR is overexpressed in gastric and duodenal neuroendocrine tumors PMID:24565507
GIPR expression was downregulated in subcutaneous adipose tissue from obese patients and correlated negatively with body mass index, waist circumference, systolic blood pressure, and glucose and triglyceride levels. PMID:24512489
GIPR promoter was hypomethylated in type 2 diabetic patients as compared to controls. PMID:24086540
This study demonstrates an association between a functional GIPR polymorphism Glu354Gln (rs1800437) and Bone mineral density and fracture risk. PMID:24446656
Compared with the current treatment standard SSTR2, GIPR has only somewhat lesser absolute gene expression in tumor tissue but much lesser expression in normal tissue, making it a promising new target for neuroendocrine tumor imaging and therapy. PMID:24238043
Structural and pharmacological characterization of novel potent and selective monoclonal antibody antagonists of glucose-dependent insulinotropic polypeptide receptor. PMID:23689510
Functional expression of a GIP receptor mutant lacking N-glycosylation is rescued by co-expressed wild type GLP1 receptor, which suggests formation of a GIP-GLP1 receptor heteromer. PMID:22412906
Our prospective, observational study indicates that the type 2 diabetes risk by dietary intake of carbohydrate and fat may be dependent on GIPR genotype. PMID:22399504
Most of the somatostatin receptor-negative neuroendocrine tumors and GLP-1 receptor-negative malignant insulinomas are GIP receptor positive. PMID:22112810
The T allele of GIPR rs2287019 is associated with greater improvement of glucose homeostasis in individuals who choose a low-fat, high-carbohydrate, and high-fiber diet. PMID:22237064
a role of the adipocyte GIPr in nutrient-dependent regulation of body weight and lean mass, but it does not support a direct and independent role for the adipocyte or beta-cell GIPr in promoting adipogenesis. PMID:22027838
Variations of the GIPR sequence are not associated with childhood obesity. PMID:20516203
Variants at the GIPR locus associated with 2-h glucose level (rs10423928, beta (s.e.m.) = 0.09 (0.01) mmol/l per A allele, P = 2.0 x 10(-15)), were identified. PMID:20081857
Proximal 5' flanking region is TATA-less and contains a GC-rich region involved in cellular expression. PMID:12530665
differences in cellular response to gastric inhibitory polypeptide(GIP) mediated by endothelin-1 may be related to differences in activation of GIP receptor splice variants PMID:12721154
Glucose-stimulated insulin secretion is maintained despite complete absence of both GIP-1 and GIP receptors. Role for incretin receptors as essential downstream targets for glucoregulatory actions of DPP-IV inhibitors. PMID:15111503
Proximal promoter of GIP-R gene contains a GC-rich region capable of binding to Sp1 and SP3. PMID:15666829
the promoter region of the GIP-R gene revealed six consensus sequences important in regulating the reporter gene activity and capable of binding to Sp1 and Sp3 transcription factors PMID:16087722
GIPR adipose tissue gene expression was significantly lower in insulin resistant obese non-diabetic women PMID:17395281
GIPR overexpression was observed in pediatric and adult adrenocortical tumors PMID:18971217
Diabetic kidney lesions of GIPRdn transgenic mice: podocyte hypertrophy and thickening of the GBM precede glomerular hypertrophy and glomerulosclerosis. PMID:19211686
Our data suggest a potential relevance of GIPR variants for obesity. PMID:19254363
Decreased TCF7L2 protein levels in type 2 diabetes mellitus correlate with downregulation of GLP-1R and GIP-R. PMID:19386626