DLL3 Recombinant Monoclonal Antibody

Code: CSB-RA882142A1HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human

Application: ELISA, FC

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Target Background

Product Details

Uniprot NO.

Q9NYJ7

Target Names

DLL3

Alternative Names

Delta-like protein 3 (Drosophila Delta homolog 3) (Delta3), DLL3

Species Reactivity

Human

Immunogen

Recombinant Human DLL3 protein

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

7B7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Flow Cytometry (FC) Protocol

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Untransfected HEK293 cells surface (green line) and transfected Human DLL3 HEK293 stable cells surface (red line) were stained with anti-DLL3 antibody (rovalpituzumab-like) (2µl/1*10⁶ cells), washed and then followed by FITC-conjugated anti-Human IgG1 Fc antibody and analyzed with flow cytometry.
The Binding Activity of DLL3 with Anti-DLL3 Recombinant Antibody.
Activity: Measured by its binding ability in a functional ELISA. Immobilized DLL3 (CSB-MP3536MOV) at 2 μg/mL can bind Anti-DLL3 Recombinant Antibody(CSB-RA882142A1HU), the EC₅₀ is 1.625-2.702 ng/mL.
The Binding Activity of DLL3 with Anti-DLL3 Recombinant Antibody.
Activity: Measured by its binding ability in a functional ELISA. Immobilized DLL3 (CSB-MP882142HU) at 2 μg/mL can bind Anti-DLL3 Recombinant Antibody(CSB-RA882142A1HU), the EC₅₀ is 1.102-1.707 ng/mL.
The purity of DLL3 was greater than 95% as determined by SEC-HPLC

Description

DLL3 (Delta-like protein 3) serves as a critical ligand in the Notch signaling pathway, playing essential roles in somite segmentation during embryonic development and neural differentiation. Unlike other Notch ligands, DLL3 functions primarily as an inhibitor of Notch signaling, making it a compelling target for researchers investigating developmental biology, neuroscience, and increasingly, cancer biology where its aberrant expression has garnered significant therapeutic interest.

This recombinant monoclonal antibody against human DLL3, clone 7B7, offers the reproducibility and consistency that demanding research applications require. Produced using recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability inherent in traditional hybridoma production, ensuring your experimental results remain comparable across studies and over time. The human IgG1 isotype format and affinity-chromatography purification deliver a reagent optimized for sensitive detection methods.

Validation studies demonstrate robust performance in both flow cytometry and ELISA applications. Flow cytometric analysis clearly distinguishes between untransfected HEK293 cells and HEK293 cells stably expressing human DLL3, confirming specific surface detection of the target protein. Functional ELISA testing reveals excellent binding characteristics, with EC50 values in the low nanogram per milliliter range against immobilized DLL3 protein, indicating high-affinity target recognition suitable for quantitative applications.

For flow cytometry experiments, recommended dilutions of 1:50 to 1:200 provide flexibility to optimize signal-to-noise ratios across different experimental conditions. Whether your research focuses on Notch pathway regulation, neural development, or cell surface marker characterization, this antibody provides a reliable tool for investigating DLL3 biology in human samples.

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Target Background

Function(From Uniprot)

Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm.

Gene References into Functions

these results reveal that DLL3 is expressed in tumor specimens from most patients with small cell lung cancer PMID:29290251
Results indicated that DLL3 expression was silenced in hepatocellular carcinoma (HCC) cells by DNA methylation and was more readily affected by histone acetylation than histone methylation (H3K9me2 or H3K27me3). PMID:29512761
our results indicated epidermal growth factor-like domain multiple 7 protein participates in growth hormone-secreting pituitary adenoma proliferation and invasion regulation via Notch2/DLL3 signaling pathway. These findings raised the possibility that epidermal growth factor-like domain multiple 7 protein might serve as a useful biomarker to assess growth hormone-secreting pituitary adenoma invasion and prognosis PMID:28705113
The Dll3 was rarely detectable in the para-carcinoma tissues, but positive in 82.1% of non-small cell cancer tissues. PMID:28007595
Both global haplotype and individual haplotype analyses showed that the haplotypes of SNP1/SNP2/SNP3/SNP4/SNP5 did not correlate with the disease (P >0.05). Together, these data suggest that genetic variants of the DLL3 gene are not associated with CS in the Chinese Han population. PMID:27472720
DLL3 was silenced by methylation in human human hepatocellular carcinoma and it negatively regulates the growth of human hepatocellular carcinoma cells. PMID:23337976
We suggest that the three human DLL3 mutations associated with spondylocostal dysplasia are also functionally equivalent to the Dll3(neo) null allele in mice. PMID:11923214
mutations in DLL3 cause a consistent pattern of abnormal vertebral segmentation in spondylocostal dysostosis PMID:12746394
no novel or previously described mutations are present in our cohort, indicating that DLL3 mutations may not be a major cause of congenital scoliosis. PMID:15717203
The intracellular region of Notch ligands Dll1 and Dll3 regulates their trafficking and signaling activity PMID:18676613