CEACAM8 Recombinant Monoclonal Antibody

Code: CSB-RA937940A0HU

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Size:

50μl100μl

US$210

Quantity:

Species Reactivity: Human, Mouse

Application: ELISA, WB

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Target Background

Product Details

Uniprot NO.

P31997

Target Names

CEACAM8

Alternative Names

Carcinoembryonic antigen-related cell adhesion molecule 8 (CD67 antigen) (Carcinoembryonic antigen CGM6) (Non-specific cross-reacting antigen NCA-95) (CD antigen CD66b), CEACAM8, CGM6

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from Human CEACAM8

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

24H12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000

Protocols

ELISA Protocol Western Blotting(WB) Protocol

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: HL60 whole cell lysate(30µg), BxPC-3 whole cell lysate(30µg), JK whole cell lysate(30µg), HepG2 whole cell lysate(30µg), HT29 whole cell lysate(30µg),THP-1 whole cell lysate(30µg),Raji whole cell lysate(30µg),A549 whole cell lysate(30µg),Mouse Spleen tissue lysate(20µg)
All lanes: CEACAM8 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 39, 70-100 kDa
Observed band size: 39,100 kDa
Exposure time: 120s

Description

CEACAM8, also known as CD66b, is a glycosylphosphatidylinositol-anchored member of the carcinoembryonic antigen family that plays a significant role in neutrophil activation and adhesion processes. As a marker predominantly expressed on granulocytes, CEACAM8 has become an important target for researchers investigating innate immune responses, inflammatory conditions, and neutrophil biology. Its involvement in cell-cell interactions and signal transduction makes it a valuable focus for immunology studies exploring leukocyte function and disease mechanisms.

This recombinant monoclonal antibody, clone 24H12, offers the consistency and reliability that demanding experimental workflows require. Produced using recombinant technology in rabbit host, this antibody provides sequence-defined specificity against a synthesized peptide derived from human CEACAM8, ensuring reproducible results across experiments and eliminating the lot-to-lot variability that can complicate long-term research projects. The affinity-chromatography purification process delivers a high-purity reagent suitable for sensitive detection applications.

Validation studies demonstrate robust performance in Western blot analysis across multiple human cell lines, including JK, HepG2, HT29, THP-1, and A549 lysates. The observed band at approximately 100 kDa aligns with the higher predicted molecular weight range, reflecting the extensive glycosylation characteristic of CEACAM family members. This antibody is also validated for ELISA and flow cytometry applications, with recommended dilutions of 1:50-1:200 for flow cytometry, providing flexibility for researchers working across different experimental platforms.

For investigators studying neutrophil biology, inflammatory responses, or immune cell characterization, this CEACAM8 antibody delivers the specificity and reproducibility essential for generating publication-quality data.

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Target Background

Function(From Uniprot)

Cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner. Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Heterophilic interaction with CEACAM8 occurs in activated neutrophils.

Gene References into Functions

Increased CD66b positive tumor-infiltrating neutrophils (CD66b + TINs) was significantly associated with presence of metastasis, S stage, and nonseminomatous germ cell tumor diagnosis. PMID:27863478
Data suggest ways in which carcinoembryonic antigen-related cell adhesion molecules CEACAM6 and CEACAM8 regulate the biological functions of one another. PMID:26483485
Granulocytes from type 2 diabetes patients display higher granulocyte cell surface expression of CD66b compared with values of healthy controls. PMID:23686079
The highly elevated gene expression of CEACAM6 and CEACAM8 in primary myelofibrosis can serve as molecular markers of myelofibrotic transformation. PMID:21470677
All the leukemic samples showed overexpression of CEACAM6 and 8 when compared with normal granulocytes. PMID:17909799
CD66b molecules are involved in regulating adhesion and activation of eosinophils, possibly through their localization in lipid rafts and interaction with other cell surface molecules, such as CD11b. PMID:18056392