ARG1 Recombinant Monoclonal Antibody

Code: CSB-RA042122A0HU

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Size:

50μl100μl

US$210

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Species Reactivity: Human

Application: ELISA, IHC

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Target Background

Product Details

Uniprot NO.

P05089

Target Names

ARG1

Alternative Names

Arginase-1 (EC 3.5.3.1) (Liver-type arginase) (Type I arginase), ARG1

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human ARG1

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

31D12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Scope Application

ELISA Protocol Immunohistochemistry (IHC) Protocol

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

IHC image of CSB-RA042122A0HU diluted at 1:50 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.71% DAB.

Description

Arginase-1 serves as a critical enzyme in the urea cycle, catalyzing the hydrolysis of arginine to ornithine and urea in hepatocytes. Beyond its metabolic function, ARG1 has emerged as a significant marker in immunology research, where its expression in myeloid-derived suppressor cells and tumor-associated macrophages contributes to immunosuppressive microenvironments by depleting arginine availability for T cell proliferation. This dual role in metabolism and immune regulation makes ARG1 a valuable target for researchers investigating liver biology, cancer immunology, and metabolic disorders.

This recombinant monoclonal antibody, clone 31D12, offers the reproducibility that demanding research protocols require. Generated against a synthetic peptide derived from human ARG1 and produced using recombinant technology, this antibody provides sequence-defined consistency between lots, eliminating the variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format ensures robust signal amplification with standard secondary detection systems.

Validation in immunohistochemistry demonstrates reliable performance in formalin-fixed, paraffin-embedded human liver cancer tissue, with recommended working dilutions of 1:50 to 1:200 providing flexibility to optimize signal-to-background ratios for your specific tissue processing conditions. The citrate buffer antigen retrieval protocol and overnight primary incubation at 4°C yielded clear visualization using standard HRP-polymer detection, confirming compatibility with automated staining platforms including the Leica Bond system. Additional ELISA compatibility extends the utility of this antibody across different experimental workflows.

Whether investigating hepatocellular carcinoma biology, characterizing tumor immune infiltrates, or studying metabolic pathway regulation, this ARG1 antibody provides a dependable tool for advancing your research in metabolism and signal transduction.

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Target Background

Function(From Uniprot)

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys.; Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion. In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival. In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.

Gene References into Functions

study of the significance of heat activation and the role of metal ions in human arginase PMID:30282613
TGF-beta1 and arginase-1 may play important roles in determining long-term graft survival. PMID:30074212
These results showed that arginase controlled sFlt-1 elevation to some extent. PMID:29548823
ARG1 gene polymorphisms and their association in individuals with essential hypertension in Pakistan has been presented. PMID:29756997
A subset of well-differentiated hepatocellular carcinomas are arginase-1 negative. PMID:28970136
The data in this study suggest that arginase I inhibition potentially represents a novel therapeutic target for the prevention and/or treatment of bronchopulmonary dysplasia-associated pulmonary hypertension. PMID:27895230
this study shows that infiltrating macrophages expressing Arg1 are present in active allergic contact dermatitis lesions PMID:28747341
High arginase expression is associated with glioblastoma. PMID:27006175
Report the value of Arg-1 in distinguishing HepPar-1-positive prostatic carcinoma from hepatocellular carcinoma at metastatic sites or cases of liver metastasis from prostate carcinoma. PMID:27184483
AEG-1 is positively activated in the tumorigenesis and deterioration of NSCLC. PMID:28152520
Arginase-1 expression is common (62.5%) in hepatoid adenocarcinoma and hence it is not useful in distinguishing hepatocellular carcinoma from hepatoid adenocarcinoma. PMID:27137985
Arginase 1 was highly expressed by tumor-associated Gr1+ microglia and macrophages. PMID:27936099
The authors report here that Candida albicans blocks nitric oxide production in human-monocyte-derived macrophages by induction of host arginase activity. PMID:28119468
Evidence for a negative association of arginase I with job strain and positive association with job control and social support in females. PMID:28403218
Two argininemia patients were initially diagnosed by tandem mass spectrometry in newborn screening. Mutation analysis of the ARG1 gene was performed by direct sequencing.Two missense mutations, p.D100N and p.R71T, in Patient-1 were predicted to lower the stability of arginase Iota by analysis of 3D crystal structure, while two nonsense mutations, p.G12X and p.E42X, in Patient-2 were predicted to lead to truncated protein. PMID:28089752
The results of this study suggested a novel relationship exists between ARG1, neutrophil-lymphocyte ratio , and stroke severity which may help guide future mechanistic studies of post-stroke immune suppression. PMID:26515089
This study provides a molecular mechanism of the pathogenesis of systemic lupus erythematosus by demonstrating an Arg-1-dependent effect of myeloid-derived suppressor cells in the development of TH17 cell-associated autoimmunity. PMID:27009269
ARG1 rs2781659 AA and rs2781667 TT genotypes were associated with lower IIEF scores (increased severity) in clinical erectile dysfunction (ED), whereas ARG1 GTCC haplotype is associated with higher IIEF scores in clinical ED, thus suggesting a genetic contribution of ARG1 variations to ED PMID:26537638
These results showed that alterations in the expression levels of Arg I and iNOS in the peripheral T cells and peripheral nodes of HIV infected patients are associated with disease progression in these patients. PMID:26647762
Increased ARG1 expression in macrophages after a single radiotherapy dose is an independent prognostic factor of skin toxicities. PMID:26061397
Arginase inhibition arrests human pulmonary artery smooth muscle cells in the G1/G0-phase under hypoxic conditions. PMID:26126810
Arginase from neutrophils can modulate nitric oxide production from activated macrophages which may affect the course of infection by intracellular bacteria. PMID:26119192
Overexpression and elevated activity of arginase I are involved in tobacco-induced pulmonary endothelial dysfunction. PMID:25889611
This method not only solved the problem of obtaining a large amount of arginase, but also provided a promising alternative for the future industrial production of L-Orn. PMID:26227111
the combination of high levels of CD14, FOXP3, and ARG1 mRNAs identified a small group of patients with excellent event-free and overall survival. PMID:26161395
Overexpression of Arg1 in the CNS of transgenic mice significantly reduced tau pathology. PMID:26538654
data indicate that helminth coinfection induces arginase-1-expressing type 2 granulomas, thereby increasing inflammation and TB disease severity. PMID:26571397
The data exclude a prognostic role of IL-10 and ARG-1 in metastatic neuroblastoma. PMID:25961062
Arginase activity increases in peripheral blood of patients with intestinal schistosomiasis. PMID:25786588
Data indicate that arginase-1 showed positivity in 2 ampullary region carcinomas and diffuse positivity in 1 duodenal adenocarcinoma. PMID:26030248
Arg1 induced accumulation of autophagosomes in MDA-MB-231 cells. PMID:25501824
Arg1 and PD-L1 are dynamically modulated upon neutrophil migration into human airways, and, Arg1, but not PD-L1, contributes to early neutrophil-driven T cell suppression in cystic fibrosis, likely hampering resolution of infection and inflammation. PMID:25926674
These results suggest that ARG1 and GABA influence both neural development and neuroblastoma and that benzodiazepines in clinical use may have potential applications for neuroblastoma therapy. PMID:25437558
Arg1 expression is decreased, and Arg2 expression is increased in the newborn congenital obstructive nephropathy and in the mouse model. PMID:25205225
rs2781666 may be associated with protection against pulmonary hypertension in preterm neonates with bronchopulmonary dysplasia PMID:24919409
The plasma levels of arginase I was higher in patients with DCL. PMID:25124926
Novel variants in the ARG1 locus associated with CRP levels in cardiovascular disease in a Korean population. [Meta-analysis] PMID:24763700
Arginase I levels are decreased in the plasma of pediatric patients with atopic dermatitis. PMID:25027824
Arginase activity was higher in cord blood of gestational diabetes mellitus mothers as opposed to the control group. PMID:24376824
Our results suggest that serum ARG and CRP together can efficiently diagnose Head and neck squamous cell carcinoma. PMID:24715304
Serum arginase I might regulate serum L-arginine and 3-nitrotyrosine via L-arginine. PMID:24060156
The independent associations of arginase I with urinary 8-OHdG and serum insulin may reflect its involvement in oxidative stress and diabetes mellitus. PMID:24005081
Arginase-1 mRNA expression correlates with myeloid-derived suppressor cell levels in peripheral blood of NSCLC patients. PMID:23850196
Both arginase-1 and HepPar-1 are effective markers of hepatocellular differentiation PMID:24281232
This study demonistrated that Five novel mutations in ARG1 gene in Chinese patients of argininemia. PMID:23859858
Glypican 3 and arginase-1 are the most reliable markers for identifying scirrhous hepatocellular carcinoma. PMID:23348905
Enzymes that are directly involved in the formation of urea are expressed in ocular tissues. PMID:23740519
Results show that the positively charged state of arginine is stable in the active site of arginase I, with that stabilization facilitated by the presence of hydroxide. PMID:23327293
The tumor suppressive function of arginase-I in both infiltrating and circulating myeloid-derived suppressor cells is a downstream target of activated STAT3. PMID:23454751
results suggest that Arg-1 may play a tumor suppressive role in HCC and could be a new, promising prognostic biomarker for HCC patients PMID:23505904

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Subcellular Location

Cytoplasm. Cytoplasmic granule.

Protein Families

Arginase family

Tissue Specificity

Within the immune system initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMNs]). Also detected in macrophages mycobacterial granulomas. Expressed in group2 innate lymphoid cells (ILC2s) during lung disease.

Database Links

HGNC: 663

UNIGENE: Hs.440934

KEGG: hsa:383

STRING: 9606.ENSP00000357066

OMIM: 207800

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