Home
Product
Protein
Recombinant Proteins
Recombinant Drosophila melanogaster Polycomb protein PHO (pho), partial

Recombinant Drosophila melanogaster Polycomb protein PHO (pho), partial

Code
MSDS	MSDS Datasheet
Size	Pls inquire
Source
Conjugate
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Abbreviation

pho

Purity

>85% (SDS-PAGE)

Target Names

pho

Uniprot NO.

Q8ST83

Alternative Names

pho; CG17743; Polycomb protein PHO; Protein pleiohomeotic; Transcription factor YY1 homolog

Species

Drosophila melanogaster (Fruit fly)

Source

Yeast

Expression Region

1-520

Target Protein Sequence

MAYERFGIIL QSEQYDEDIG NTKVNQKMNE GNHYDLHRKN AFDRIIHSES KKGDNVINYN IHENDKIKAA DNIFSSKLKM NPNMSYEMNI NCFKNIGYGE NQETSKVLTN SLSNNDINTE ESGVVDKNSP FLTLGTTILN SNGKSRRWEQ KLVHIKTMEG EFSVTMWASG ISDDEYSGSD QIVGASDLLK GKEEFGIDGF TSQQNKEYQK MESKFTNAQT LEMPHPISSV QIMDHLIKER GNLSQENNIS ERILSKTTLS FEEPILLPDS SSIELVNETA AMTINNHRTL SNHTGNTGDL HALPSSVPFR IGLHEGQVND CLSTISQSTH QDNTDSTGCG EMNLSEVTVS YTNDKKIACP HKGCNKHFRD SSAMRKHLHT HGPRVHVCAE CGKAFVESSK LKRHQLVHTG EKPFQCTFEG CGKRFSLDFN LRTHVRIHTG DRPFVCPFDA CNKKFAQSTN LKSHILTHAK AKRNTSISGK SGCSNAESNS QSEDTSANYV KVELQDSVTE NHVPFVVYAD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Protein Length

Partial

Tag Info

N-terminal His-tagged/Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Storage Condition

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Shelf Life

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Customer Reviews and Q&A

■ Customer Reviews

Submit a Review here

Target Background

Function(From Uniprot)

Polycomb group (PcG) protein that binds to the 5'-CNGCCATNNNNG-3' sequence found in the regulatory regions of many genes. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Probably targets the Esc/E(z) complex to DNA. Necessary but not sufficient to recruit a functional PcG repressive complex that represses target genes, suggesting that the recruitment of the distinct PRC1 complex is also required to allow a subsequent repression.; Proposed core component of the chromatin remodeling Ino80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.

Gene References into Functions

Mutation in the protein-protein interaction domain (REPO domain) of Pho interferes with the dynamics of its interaction with Spt5. The transcriptional kinetics of the heat shock response is negatively affected by a mutation in the REPO domain of Pho PMID:29208012
Spt5 interacts both physically and genetically with the Polycomb Group (PcG) protein Pleiohomeotic (Pho) PMID:23894613
The TF homolog pair Pho/Yy1 seems to play very different roles in PcG targeting between Drosophila and mouse. PMID:20353814
Pleiohomeotic protein has a role in polycomb response element-mediated silencing for transcriptional repression PMID:12370294
This protein functions as a PcG protein in vivo. PMID:12628927
The mammalian transcription factor YY1 can function as a PcG protein in Drosophila resulting in transcriptional repression and correction of phenotypic defects caused by mutation of its homologue, Pleiohomeotic (PHO). PMID:16052488
Pleiohomeotic (PHO) and a multiprotein Polycomb core complex (PCC) bind highly cooperatively to polycomb response elements PMID:16077005
grainyhead interacts genetically with pleiohomeotic in a transgene-based, pairing-dependent silencing assay PMID:16449654
YY1 sequences 201-226 are necessary and sufficient for Polycomb group transcriptional repression in vivo. PMID:17158804
These results suggest that pho functions in maintaining expression of genes involved in the formation of ventral appendages and the proximo-distal axis. PMID:18495104
Pho is a general component of the maintenance machinery, since the majority of Pc targets are also associated with Pho (Pleiohomeotic )binding. PMID:18773083
a DNA-binding complex requiring Pho is differentially regulated to facilitate epigenetic transcriptional memory of both the active and the repressed state PMID:19029043