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Phosphate Buffer

Phosphate Buffer

（1）Na2HPO4-NaH2PO4 Buffer

Stocks solutions
  • A: Disodium Hydrogen Phosphate (Na2HPO4 • 2H2O mw: 178.05 g/mol)
  • B: Sodium Dihydrogen Phosphate (NaH2PO4 • 2H2O mw: 156.01 g/mol)
  • C: Distilled water
To prepareL of Na2HPO4-NaH2PO4 Buffer ( M, pH )

Input buffer volume, pH, molar concentration to get formula.

Table 1. Required components

Component Mass（g）
A (Disodium Hydrogen Phosphate, mw: 178.05 g/mol) A
B (Sodium Dihydrogen Phosphate, mw: 156.01 g/mol)) B
Steps：
  • Prepare C mL of distilled water in a suitable container.
  • Add A g of Disodium Hydrogen Phosphate to the solution.
  • Add B g of Sodium Dihydrogen Phosphate to the solution.
  • Adjust solution to final desired pH using HCl or NaOH
  • Add distilled water until volume is X L.

（2）Na2HPO4- KH2PO4 Buffer

Stocks solutions
  • A: Disodium Hydrogen Phosphate (Na2HPO4 • 2H2O mw: 178.05 g/mol)
  • B: Monopotassium Phosphate (KH2PO4 mw: 136.09 g/mol)
  • C: Distilled water
To prepareL of Na2HPO4- KH2PO4 Buffer ( M, pH )

Input buffer volume, pH, molar concentration to get formula.

Table 1. Required components

Component Mass（g）
A (Disodium Hydrogen Phosphate, mw: 178.05 g/mol ) A
B (Monopotassium Phosphate, mw: 136.09 g/mol)) B
Steps：
  • Prepare C mL of distilled water in a suitable container.
  • Add A g of Disodium Hydrogen Phosphate to the solution.
  • Add B g of Monopotassium Phosphate to the solution.
  • Adjust solution to final desired pH using HCl or NaOH
  • Add distilled water until volume is X L.
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