Phosphate-Citrate Buffer


Phosphate-Citrate Buffer
Stocks solutions
  • A: Disodium Hydrogen Phosphate (Na2HPO4 mw: 141.98 g/mol)
  • B: Citric Acid (C6H8O7H2O mw: 210.14 g/mol)
  • C: Distilled water
To prepareL of Phosphate-Citrate Buffer ( pH )

Input buffer volume, pH, molar concentration to get formula.

Table 1. Required components

Component Mass(g)
A (Disodium Hydrogen Phosphate, mw: 141.98 g/mol) A
B (Citric Acid, mw: 210.14 g/mol)) B
Steps:
  • Prepare C mL of distilled water in a suitable container.
  • Add A g of Disodium Hydrogen Phosphate to the solution.
  • Add B g of Citric Acid to the solution.
  • Adjust solution to final desired pH using HCl or NaOH
  • Add distilled water until volume is X L.
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