NTSR2 Antibody

Code: CSB-PA016138LA01HU

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US$83

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Species Reactivity: Human

Raised in: Rabbit

Application: ELISA, WB, IHC, IF

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Product Details
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Target Background

Product Details

Uniprot NO.

O95665

Target Names

NTSR2

Alternative Names

NTSR2; Neurotensin receptor type 2; NT-R-2; NTR2; Levocabastine-sensitive neurotensin receptor

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Neurotensin receptor type 2 protein (359-410AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: U87 whole cell lysate
All lanes: NTSR2 antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA016138LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA016138LA01HU at dilution of 1:100
Immunofluorescence staining of Hela cells with CSB-PA016138LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Customer Reviews and Q&A

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Target Background

Function(From Uniprot)

Receptor for the tridecapeptide neurotensin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Gene References into Functions

We showed that expression levels for NTS and NTSR1 varied, that NTSR2 expression was not detectable and that NTSR3 was consistently expressed in all CRC cell lines examined. PMID:28498396
high expression of NTSR1 is found in clinical NETs and promoter methylation is an important mechanism controlling the differential expression of NTSR1 and silencing of NTSR2 in NET cells PMID:26298774
NTSR2 was overexpressed, NTSR1 decreased, and neurotensin was not expressed in B cell leukemia patient's B-cells, as compared with healthy B cells. PMID:23109725
this study proposes a novel function of NTR2 in the regulation of NTR1 activity. PMID:19968961
NTR2 and NTR3 were upregulated in prostate cancer cells with luminal phenotype (cytokeratin 18+) PMID:20048080
Recycling ability depends on a single tyrosine residue PMID:11801734
ghrelin receptor, neurotensin receptor 2 and GPR39 display an unusually high degree of constitutive activity, determined by an aromatic cluster on the inner face of the extracellular ends of TMs VI and VII PMID:15383539
NTR1 and NTR2 mRNA were not detected in either pituitary adenomas or normal tissue. PMID:18624930