| Application | Recommended Dilution |
|---|---|
| WB | 1:1000-1:5000 |
| IHC | 1:20-1:200 |
Western blot
All lanes: Hras antibody at 2µg/ml
Lane 1: 293T whole cell lysate
Lane 2: Mouse brain tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Immunohistochemistry of paraffin-embedded human lymphoid tissue using CSB-PA15539A0Rb at dilution of 1:100
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