FUNDC1 Antibody

Code: CSB-PA816886LA01HU

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Size:

50ul100ul

US$166

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Species Reactivity: Human, Mouse, Rat

Raised in: Rabbit

Application: ELISA, WB, IHC

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Product Details
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Target Background

Product Details

Uniprot NO.

Q8IVP5

Target Names

FUNDC1

Alternative Names

FUNDC1; FUN14 domain-containing protein 1

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human FUN14 domain-containing protein 1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:500

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Datasheet & COA

Antibody FAQs

Images

Western Blot
Positive WB detected in: RAW264.7 whole cell lysate(30µg), 293 whole cell lysate(30µg), SY5Y whole cell lysate(30µg), Hela whole cell lysate(30µg), MCF7 whole cell lysate(30µg)
All lanes: FUNDC1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 17kDa
Observed band size: 17kDa
Exposure time: 60s
IHC image of CSB-PA816886LA01HU diluted at 1:250 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
Western Blot
Positive WB detected in: Mouse Brain tissue lysate(30µg),Mouse Liver tissue lysate(30µg),Rat Brain tissue lysate(30µg),Rat Liver tissue lysate(30µg)
All lanes: FUNDC1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 60s
IHC image of CSB-PA816886LA01HU diluted at 1:250 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
IHC image of CSB-PA816886LA01HU diluted at 1:250 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

Description

The FUNDC1 antibody was derived from the rabbits immunized with the recombinant human FUNDC1 protein (1-46aa). This rabbit anti-human FUNDC1 polyclonal antibody was demonstrated to recognize human, mouse, and rat FUNDC1 proteins in ELISA and IHC applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity.

The target protein FUNDC1 mainly regulates mitophagy by serving as a receptor that recruits damaged or dysfunctional mitochondria to the autophagosome for degradation. It is also involved in regulating mitochondrial morphology and dynamics, as well as various physiological and pathological processes.

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Target Background

Function(From Uniprot)

Acts as an activator of hypoxia-induced mitophagy, an important mechanism for mitochondrial quality control.

Gene References into Functions

Our data suggested that FUNDC1 can be used as a prognostic biomarker in patients with cervical cancer, and may be a new therapeutic target to improve the antitumor effects of chemoradiotherapy. PMID:28719148
phosphorylation of Tyr18 of FUNDC1 serves as a molecular switch for mitophagy. PMID:27653272
FUNDC1 integrates mitochondrial fission and mitophagy at the interface of the endoplasmic reticulum-mitochondrial contact site by working in concert with DRP1 and calnexin under hypoxic conditions in mammalian cells. PMID:27145933
FUNDC1 regulates both mitochondrial fission or fusion and mitophagy. PMID:27050458
The BCL2L1-PGAM5-FUNDC1 axis is critical for receptor-mediated mitophagy. PMID:25126723
FUNDC1 regulates ULK1 recruitment to damaged mitochondria, where FUNDC1 phosphorylation by ULK1 is crucial for mitophagy. PMID:24671035
Data show that Knockdown of endogenous FUNDC1 significantly prevented hypoxia-induced mitophagy, which could be reversed by the expression of wild-type FUNDC1. PMID:22267086